DLG5 Antibody - BSA Free

Immunoprecipitation: DLG5 Antibody [NBP1-28694] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DLG5 antibody NBP1-28694 used for IP at 10 ug/mg lysate. DLG5 was also immunoprecipitated by rabbit anti-DLG5 antibody NBP1-28739, which recognizes a downstream epitope. For blotting immunoprecipitated DLG5, NBP1-28739 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DLG5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DLG5 Antibody - BSA Free (NBP1-28694) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 800 and 850 of human discs, large homolog 5 using the numbering given in entry NP_004738.3 (GeneID 9231).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLG5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
• Application Notes: It is not recommended for western analysis.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DLG5 Antibody - BSA Free

• discs, large homolog 5 (Drosophila)
• KIAA0583large (Drosophila) homolog 5
• LP-DLG

Background

DLG5 (discs large homolog 5) is a member of the family of discs large (DLG) homologs, a subset of the membrane-associated guanylate kinase (MAGUK) superfamily. The MAGUK proteins are composed of a catalytically inactive guanylate kinase domain, in addition to PDZ and SH3 domains, and are thought to function as scaffolding molecules at sites of cell-cell contact. DLG5 localizes to the plasma membrane and cytoplasm, and interacts with components of adherens junctions and the cytoskeleton. It is proposed to function in the transmission of extracellular signals to the cytoskeleton and in the maintenance of epithelial cell structure. [from NCBI GeneID:9231]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLG5
• Entrez:
  • 9231(Human)
• Uniprot:
  • NP_004738.3(Human)