Dkk-3 Antibody (8A5C6) - BSA Free

Western Blot: Dkk-3 Antibody (8A5C6) [NBP2-37430] - Western blot analysis using DKK3 mAb against HEK293 (1) and DKK3 (AA: 91-350)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: Dkk-3 Antibody (8A5C6) [NBP2-37430] - Analysis of brain tissues using DKK3 mouse mAb with DAB staining.

Western Blot: Dkk-3 Antibody (8A5C6) [NBP2-37430] - Western blot analysis using DKK3 mAb against human DKK3 recombinant protein. (Expected MW is 54.4 kDa)

ELISA: Dkk-3 Antibody (8A5C6) [NBP2-37430] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, CyTOF-ready
• Clone: 8A5C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Dkk-3 Antibody (8A5C6) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human DKK3 (AA: 91-350 ) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DKK3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 38.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Dkk-3 Antibody (8A5C6) - BSA Free

• dickkopf (Xenopus laevis) homolog 3
• dickkopf 3
• dickkopf homolog 3 (Xenopus laevis)
• Dickkopf-3
• dickkopf-related protein 3
• Dkk3
• Dkk-3
• hDkk-3
• regulated in glioma
• REIC
• REICdkk-3
• RIG
• RIG-like 5-6
• RIG-like 7-1

Background

DKK3 is a secreted protein that is a member of the dickkopf family. It contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dkk-3 Antibody (NBP2-37430)(1)

Publication using NBP2-37430

• Peng X, Lai K, She P et al. Induction of Wnt signaling antagonists and P21-activated kinase enhances cardiomyocyte proliferation during zebrafish heart regeneration J Mol Cell Biol

Bioinformatics

• Gene Symbol: DKK3