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VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
1118-DK) was serially diluted and captured by Mouse Anti-Human Dkk‑3 Monoclonal Antibody (Catalog # MAB11181) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Dkk‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1118) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
DKK3 is a secreted protein that is a member of the dickkopf family. It contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.
![Immunohistochemistry Dkk-3 Antibody [Unconjugated]](http://images.novusbio.com/images/af1118_human-dkk-3-affinity-purified-polyclonal-ab-immunohistochemistry-26102022101347.jpg)
![Western Blot Dkk-3 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af948_mouse-dkk-3-affinity-purified-polyclonal-ab-western-blot-12122025748130.jpg)
![Sandwich ELISA Dkk-3 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/Dkk-3-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79549-img0001.jpg)
![Sandwich ELISA Dkk-3 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/Dkk-3-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79668-img0001.jpg)
![N/A Dkk-3 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Dkk3_DY1118_ELISA_1576.jpg)
![Immunohistochemistry Dkk-3 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1118_human-dkk-3-affinity-purified-polyclonal-ab-8220249505964.jpg)
![Western Blot Dkk-3 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1118_human-dkk-3-affinity-purified-polyclonal-ab-8220249505942.jpg)
![Immunocytochemistry/ Immunofluorescence Dkk-3 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1118_human-dkk-3-affinity-purified-polyclonal-ab-8220249503213.jpg)
![Immunohistochemistry Dkk-3 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1118_human-dkk-3-affinity-purified-polyclonal-ab-8220249505930.jpg)
![Western Blot Dkk-3 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1118_human-dkk-3-affinity-purified-polyclonal-ab-822024951142.jpg)
![Immunohistochemistry Dkk-3 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1118_human-dkk-3-affinity-purified-polyclonal-ab-8220249503284.jpg)
![Western Blot Dkk-3 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af1118_human-dkk-3-affinity-purified-polyclonal-ab-44202415425069.jpg)
![ELISA Dkk-3 Antibody [Unconjugated]](http://images.novusbio.com/images/af1118_human-dkk-3-affinity-purified-polyclonal-ab-elisa-639201854766295512.jpg)
