Human Dkk-1 ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA1869
    • Availability
      Product Discontinued

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Human Dkk-1 ELISA Kit (Colorimetric) Summary

Sensitivity
100 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
DKK1

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
DKK1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DKK1.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culturemedium/urine) diluent.
  2. Assay Diluent D (Item K): 15 ml of 5x concentrated buffer. For Standard/Sample (serum/plasma) diluent.
  3. Detection Antibody Dkk-1 (Item F): 2 vial of biotinylated anti-human Dkk-1 (each vial is enough to assay half microplate).
  4. Dkk-1 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human Dkk-1.
  5. HRP-Streptavidin Concentrate (Item G): 8 µl 35,000x concentrated HRP-conjugated streptavidin.
  6. Standards (Item C): 2 vials of recombinant human Dkk-1.
  7. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  8. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution.
  9. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Dkk-1 ELISA Kit (Colorimetric)

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Background

DKK1 is a secreted factor that has been linked to the function of osteoblasts. Elevated DKK1 levels in bone marrow plasma and peripheral blood from patients with multiple myeloma correlated with the gene-expression patterns of DKK1 and were associated with the presence of focal bone lesions. The production of DKK1, an inhibitor of osteoblast differentiation, by myeloma cells is associated with the presence of lytic bone lesions in patients with multiple myeloma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Dkk-1 ELISA Kit (KA1869). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol DKK1