Human Dkk-1 ELISA Kit (Colorimetric)

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ELISA: Human Dkk-1 ELISA Kit (Colorimetric) [KA1869] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Culture Supernatant, Plasma, Serum, Urine
Sensitivity
100 pg/mL

Order Details

Human Dkk-1 ELISA Kit (Colorimetric) Summary

Sensitivity
100 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 15%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
DKK1

Applications/Dilutions

Application Notes
DKK1 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human DKK1.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. HRP-Streptavidin Concentrate (Item G): 8 µl 35,000x concentrated HRP-conjugated streptavidin.
  2. Standards (Item C): 2 vials of recombinant human Dkk-1.
  3. Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
  4. Assay Diluent D (Item K): 15 ml of 5x concentrated buffer. For Standard/Sample (serum/plasma) diluent.
  5. Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culturemedium/urine) diluent.
  6. Detection Antibody Dkk-1 (Item F): 2 vial of biotinylated anti-human Dkk-1 (each vial is enough to assay half microplate).
  7. Dkk-1 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human Dkk-1.
  8. Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
  9. TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Dkk-1 ELISA Kit (Colorimetric)

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol DKK1
Entrez