Dkk-1 Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Mu
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 488

Dkk-1 Antibody [Alexa Fluor™ Plus 488] Summary

• Specificity: Detects mouse Dkk-1 in ELISAs and Western blots. In sandwich ELISAs, less than 6% cross-reactivity with recombinant human Dkk‑1 and less than 0.1% cross-reactivity with recombinant mouse (rm) Dkk-2, rmDkk-3, and rmDkk-4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dkk-1 Antibody [Alexa Fluor™ Plus 488]

• dickkopf (Xenopus laevis) homolog 1
• dickkopf homolog 1 (Xenopus laevis)
• dickkopf related protein-1
• Dickkopf-1
• dickkopf-related protein 1
• Dkk1
• Dkk-1
• hDkk-1
• SKdickkopf-1 like

Background

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk‑1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low‑density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk-1/LRP6/Krm2 complex internalization has been shown to downregulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

1. Glinka, A. et al. (1998) Nature 391:357.  
2. Niehrs, C. (2006) Oncogene 25:7469. 
3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
4. Mao, B. et al. (2001) Nature 411:321. 
5. Mao, B. et al. (2002) Nature 417:664.  
6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
8. Li, J. et al. (2006) Bone 36:754.
9. Verani, R. et al. (2006) J. Neurochem. 101:242.
10. Pinzone, J.J. et al. (2009) Blood 113:517.
11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.