Dkk-1 Antibody (SC06-86)

Western Blot: Dkk-1 Antibody (SC06-86) [NBP2-67607] - Western blot analysis of Dkk-1 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra

Immunocytochemistry/Immunofluorescence: Dkk-1 Antibody (SC06-86) [NBP2-67607] - Staining DKK1 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Dkk-1 Antibody (SC06-86) [NBP2-67607] - Analysis of paraffin-embedded human uterus tissue using anti-DKK1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Dkk-1 Antibody (SC06-86) [NBP2-67607] - Analysis of Hela cells with DKK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Dkk-1 Antibody (SC06-86) [NBP2-67607] - Analysis of DKK1 on MCF-7 cells lysates using anti-DKK1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Dkk-1 Antibody (SC06-86) [NBP2-67607] - Staining DKK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Dkk-1 Antibody (SC06-86) [NBP2-67607] - Staining DKK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Dkk-1 Antibody (SC06-86) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Dkk-1 aa 225-266 / 266. (SwissProt: O94907 Human)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DKK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Dkk-1 Antibody (SC06-86)

• dickkopf (Xenopus laevis) homolog 1
• dickkopf homolog 1 (Xenopus laevis)
• dickkopf related protein-1
• Dickkopf-1
• dickkopf-related protein 1
• Dkk1
• Dkk-1
• hDkk-1
• SKdickkopf-1 like

Background

DKK1 is a secreted factor that has been linked to the function of osteoblasts. Elevated DKK1 levels in bone marrow plasma and peripheral blood from patients with multiple myeloma correlated with the gene-expression patterns of DKK1 and were associated with the presence of focal bone lesions. The production of DKK1, an inhibitor of osteoblast differentiation, by myeloma cells is associated with the presence of lytic bone lesions in patients with multiple myeloma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DKK1