Dishevelled-3 Antibody [Unconjugated]

Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Dishevelled-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3754) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Dishevelled-3 at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Dishevelled-3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Dishevelled-3
  His545-Gly654
  Accession # Q92997
• Specificity: Detects human Dishevelled-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Dishevelled-1 and recombinant human Dishevelled-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DVL3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dishevelled-3 Antibody [Unconjugated]

• dishevelled 3 (homologous to Drosophila dsh)
• dishevelled, dsh homolog 3 (Drosophila)
• Dishevelled3
• Dishevelled-3
• DSH homolog 3
• DVL3
• KIAA0208dishevelled-3
• segment polarity protein dishevelled homolog DVL-3

Background

Dishevelled-3 is one of three mammalian multi-moduled Dishevelled proteins that have important roles in the canonical and non-canonical Wnt pathways. Human Dishevelled-3 is a 716 amino acid residue protein with an N-terminal DIX domain, a central PZD domain, and a DEP domain. Over the region used as immunogen, human Dishevelled-3 shares 99% amino acid sequence homology with mouse and canine Dishevelled-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DVL3
• Uniprot:
  • Q92997()