DIDO1 Antibody

Product Discontinued

DIDO1 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 154-198 of Human Dio-1.
• Specificity: Dio-1 (S186) pAb detects endogenous levels of Dio-1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DIDO1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Dio-1 (S186) pAb in extracts from 293 cells.
  Immunohistochemistry (IHC) analyzes of Dio-1 (S186) pAb in paraffin-embedded human colon carcinoma tissue. Flow data from customer review.
• Theoretical MW: 244 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DIDO1 Antibody

• BYE1
• C20orf158
• DATF1
• DATF-1
• DATF1DIDO3
• death associated transcription factor 1
• death inducer-obliterator 1
• Death-associated transcription factor 1
• death-inducer obliterator 1
• DIDO1
• DIO1
• DIO1DIDO2
• DIO-1hDido1
• dJ885L7.8
• DKFZp434P1115
• FLJ11265
• KIAA0333chromosome 20 open reading frame 158
• MGC16140

Background

Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs. Dio-1 translocates to the nucleus, and activates apoptosis during limb development. Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development. Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced. This process is similar to the expression of NFkappaB and NGF in response to external signals. Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression. This supports the theory that Dio-1 functions in the onset of programmed cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DIDO1 Antibody (NBP1-19706)(2)

Publications using NBP1-19706

• Porings AS, Lowin T, Dufner B et al. A thyroid hormone network exists in synovial fibroblasts of rheumatoid arthritis and osteoarthritis patients Sci Rep [PMID: 31519956] (IHC-Fr, Human)
• Wang S, Xiang C, Mou L et al. Trans-acting nonsynonymous variant of FOXA1 predisposes to hepatocellular carcinoma through modulating FOXA1-ER alpha transcriptional program and may have undergone natural selection Carcinogenesis 2019-08-11 [PMID: 31400761]

Review for DIDO1 Antibody (NBP1-19706) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Guanhua Xie Flow Mouse 08/15/2014

Summary

• Application: Flow Cytometry
• Species: Mouse

Bioinformatics

• Gene Symbol: DIDO1
• Entrez:
  • 1733(Human)
  • 13370(Mouse)
• Uniprot:
  • Q9BTC0(Human)