DIDO1 Antibody

Images

 
Western Blot: DIDO1 Antibody [NBP1-19706] - Analysis of Dio-1 (S186) pAb at 1:500 dilutionLane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:H9C2 cell lysate
Immunohistochemistry-Paraffin: DIDO1 Antibody [NBP1-19706] - Paraffin-embedded human breast carcinoma tissue at 1:50.
Flow Cytometry: DIDO1 Antibody [NBP1-19706] - Analysis on mouse hepatic stellate cells. Image from verified customer review.
Immunohistochemistry-Paraffin: DIDO1 Antibody [NBP1-19706] - Paraffin-embedded human colon carcinoma tissue.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-19706
    • Availability
      Product Discontinued

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DIDO1 Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 154-198 of Human Dio-1.
Specificity
Dio-1 (S186) pAb detects endogenous levels of Dio-1 protein.
Clonality
Polyclonal
Host
Rabbit
Gene
DIDO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Dio-1 (S186) pAb in extracts from 293 cells.
Immunohistochemistry (IHC) analyzes of Dio-1 (S186) pAb in paraffin-embedded human colon carcinoma tissue. Flow data from customer review.
Theoretical MW
244 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-19706 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DIDO1 Antibody

  • BYE1
  • C20orf158
  • DATF1
  • DATF-1
  • DATF1DIDO3
  • death associated transcription factor 1
  • death inducer-obliterator 1
  • Death-associated transcription factor 1
  • death-inducer obliterator 1
  • DIDO1
  • DIO1
  • DIO1DIDO2
  • DIO-1hDido1
  • dJ885L7.8
  • DKFZp434P1115
  • FLJ11265
  • KIAA0333chromosome 20 open reading frame 158
  • MGC16140

Background

Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs. Dio-1 translocates to the nucleus, and activates apoptosis during limb development. Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development. Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced. This process is similar to the expression of NFkappaB and NGF in response to external signals. Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression. This supports the theory that Dio-1 functions in the onset of programmed cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DIDO1 Antibody (NBP1-19706) (0)

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Review for DIDO1 Antibody (NBP1-19706) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-19706:
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Images Ratings Applications Species Date Details
Flow Cytometry DIDO1 NBP1-19706
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reviewed by:
Guanhua Xie
Flow Mouse 08/15/2014
View

Summary

ApplicationFlow Cytometry
SpeciesMouse

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Video Protocols

WB Video Protocol
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Recent Reviews

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Guanhua Xie
08/15/2014
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol DIDO1
Entrez
Uniprot