DIDO1 Antibody

Western blot shows lysates of Raji human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human DIDO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6947) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DIDO1 at approximately 270 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

DIDO1 Antibody Summary

• Immunogen: E. coli-derived recombinant human DIDO1
  Met1-Ser117
  Accession # Q9BTC0
• Specificity: Detects human DIDO1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: DIDO1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DIDO1 Antibody

• BYE1
• C20orf158
• DATF1
• DATF-1
• DATF1DIDO3
• death associated transcription factor 1
• death inducer-obliterator 1
• Death-associated transcription factor 1
• death-inducer obliterator 1
• DIDO1
• DIO1
• DIO1DIDO2
• DIO-1hDido1
• dJ885L7.8
• DKFZp434P1115
• FLJ11265
• KIAA0333chromosome 20 open reading frame 158
• MGC16140

Background

DIDO1 (Death-inducer obliterator 1; also DIO-1, C20orf158 and DATF-1) is a 245 kDa (predicted) intracellular protein that belongs the DIO family of proteins. It is ubiquitous in expression, and apparently serves multiple functions, depending on its splice variant. The standard form is a nuclear protein that maintains the integrity of mitotic checkpoint protein BubR1. An absence of DIDO1 results in centrosome amplification and unequal chromatin segregation. The best known short form (an 83 kDa isoform in mouse), by contrast, induces apoptosis by promoting transcription of caspase 9. Human standard form DIDO1 is 2240 amino acid (aa) in length. It contains two NLSs (aa 165-193), one PHD-type Zn finger region (aa 270-320), a TFIIS domain (aa 670-790), an SPOC protein-interaction domain (aa 1057-1163), two Pro-rich segments (aa 1257-1286 and 1725-2034) and a C-terminal Arg-rich region (aa 2108-2214). There are least 23 utilized phosphoryation sites. Multiple splice forms exist. Variants are generated by short aa substitutions for aa 531-2240, 566-2240 and 1182-2240, possibly coupled to a 36 aa insertion after Pro387. Over aa 1‑117, human DIDO1 shares 80% aa identity with mouse DIDO1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DIDO1 Antibody (AF6947)(1)

Publication using AF6947

• Adam H. Tencer, Jovylyn Gatchalian, Brianna J. Klein, Abid Khan, Yi Zhang, Brian D. Strahl et al. A Unique pH-Dependent Recognition of Methylated Histone H3K4 by PPS and DIDO Structure 2017-10-01 [PMID: 28919441] (Immunocytochemistry, Human)

Bioinformatics

• Gene Symbol: DIDO1
• Uniprot:
  • Q9BTC0(Human)