Dicer Antibody


Western Blot: Dicer Antibody [NB200-591] - Mouse ovary tissue lysate probed with Dicer antibody at 1:1000 dilution.

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Dicer Antibody Summary

Rabbit anti-mouse Dicer polyclonal antibody was raised against a peptide corresponding to amino acids 132-150 and 1017-1033 of mouse Dicer (EDL18787).
In mouse ovary, a 217 kDa band is observed.
Predicted Species
Rat (90%). Backed by our 100% Guarantee.
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5-3 ug/ml
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:20-1:1000
Application Notes
WB: Use at a dilution of 1/500 - 1/1000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
218.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Ovary Lysate (NB820-59664)
Read Publications using
NB200-591 in the following applications:

  • IHC
    1 publication
  • 2 publications
  • WB
    8 publications

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for Dicer Antibody

  • DCR1
  • dicer 1, double-stranded RNA-specific endoribonuclease
  • dicer 1, ribonuclease type III
  • Dicer1, Dcr-1 homolog (Drosophila)
  • EC 3.1.26
  • EC 3.1.26.-
  • EC
  • Helicase MOI
  • Helicase with RNase motif
  • helicase-moi
  • HERNADicer1, Dcr-1 homolog
  • K12H4.8-LIKE
  • KIAA0928endoribonuclease Dicer


Dicer is an RNaseIII-like enzyme which cleaves double-stranded RNA into 21-23 nt RNAs. These RNAs are referred to as siRNA (short interfering RNA). It is believed to be essential in siRNA-mediated gene suppression.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dicer Antibody (NB200-591)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Human,Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB200-591 Applications Species
Chiappinelli KB, Haynes BC, Brent MR, Goodfellow PJ. Reduced DICER1 Elicits an Interferon Response in Endometrial Cancer Cells Mol Cancer Res 2012 Feb 21 [PMID: 22252463] (WB, Human) WB Human
Grelier G, N Voirin, A Ay et al. Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype. B J Cancer 101:673-683 (2009).
Lei D, Shao Z, Zhou X, Yuan H. Synergistic neuroprotective effect of rasagiline and idebenone against retinal ischemia-reperfusion injury via the Lin28-let-7-Dicer pathway. Oncotarget. Feb 23 2018 [PMID: 29552298] (WB, Mouse) WB Mouse
Simeoli R, Montague K, Jones HR et al. Exosomal cargo including microRNA regulates sensory neuron to macrophage communication after nerve trauma Nat Commun. 2017 Nov 24 [PMID: 29176651] (WB, Mouse) WB Mouse
de Almeida DC, Bassi EJ, Azevedo H et al. A Regulatory miRNA-mRNA Network Is Associated with Tissue Repair Induced by Mesenchymal Stromal Cells in Acute Kidney Injury Front Immunol. Jan 18 2016 [PMID: 28096802] (WB, IHC, Mouse) WB, IHC Mouse
Lu XF, Zhou YJ, Zhang L et al. Loss of Dicer1 impairs hepatocyte survival and leads to chronic inflammation and progenitor cell activation World J. Gastroenterol. 2015 Jun 07 [PMID: 26074697] (IHC-P, Mouse) IHC-P Mouse
Grelier G, Voirin N, Ay AS et al. Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype. Br J Cancer. 2009 Aug 18 [PMID: 19672267] (WB, IHC-P)

1. WB: Four human breast cancer cell lines; HMEC, HMEC+hTERT, HMEC+LT+hTERT, and HMLER, Fig 2A. 2. WB: Five mouse mammary cell lines; 67NR, 168FARN, 4TO7, 66c14, and 4T1, Fig 3A. 3. IHC (Paraffin): Normal breast and ductal carcinoma tissue, Fig 4A, 4B, &
Chen JF, Murchison EP, Tang R et al. Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure. Proc Natl Acad Sci U S A. 2008 Feb 12 [PMID: 18256189]
Poleshko A, Palagin I, Zhang R et al. Identification of cellular proteins that maintain retroviral epigenetic silencing: evidence for an antiviral response. J Virol. 2008 Mar [PMID: 18094192] (WB)

WB: HeLa TI-C cells treated with Dicer siRNA, Fig 3C. Note: The (IMG-538) Dicer pAb was siRNA validated by WB in HeLa TI-C cells, Fig 3C.
Barbato C, Ciotti MT, Serafino A et al. Dicer expression and localization in post-mitotic neurons. Brain Res. 2007 Oct 17 [PMID: 17888888] (WB, Rat)

Rat neuronal primary cultures from 8 day old Wistar rats. IF (Figs 1, 2B, 2C, 3A, 4-8); WB (Figs 2A, 7A). Notes: 1. Dicer was detected as a single band just below the 220 kDa marker in total cell lysates from hippocampal, cortical, and cerebellar granule
WB Rat
Show All 15 Publications.

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Product General Protocols

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FAQs for Dicer Antibody (NB200-591). (Showing 1 - 1 of 1 FAQs).

  1. Does this antibody work for immunofluorescence staining in mouse tissue?
    • This antibody lists both Mouse and IF. We guarantee all of our products to work in all species and applications listed.

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Blogs on Dicer.

Slicing and Dicing RNA with Dicer
Dicer is an RNaseIII-like enzyme capable of cleaving double-stranded RNA (dsRNA) into smaller 21-23 nt RNA fragments known as short interfering RNA (siRNAs). It targets the selective degradation of complementary RNAs in a posttranscriptional gene sile...  Read full blog post.

Ago2 antibodies and dicer-independent biogenesis of miRNA
Ago2, also called eIF2C2, antibody is one of 37 reagents targeted to the Argonaute protein family that we at Novus Biologicals have in our antibody catalogue. Argonaute proteins are encoded by genes which play an important role in regulating the contr...  Read full blog post.

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Gene Symbol DICER1