Dicer Antibody

Images

 
Western Blot: Dicer Antibody [NB200-591] - Mouse ovary tissue lysate probed with Dicer antibody at 1:1000 dilution.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Dicer Antibody Summary

Immunogen
Rabbit anti-mouse Dicer polyclonal antibody was raised against a peptide corresponding to amino acids 132-150 and 1017-1033 of mouse Dicer (EDL18787).
Localization
IMG-538
Specificity
In mouse ovary, a 217 kDa band is observed.
Predicted Species
Rat (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DICER1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-3 ug/ml
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:20-1:1000
Application Notes
WB: Use at a dilution of 1/500 - 1/1000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
218.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Ovary Lysate (NB820-59664)
Publications
Read Publications using
NB200-591 in the following applications:

  • IHC
    1 publication
  • 2 publications
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Dicer Antibody

  • DCR1
  • dicer 1, double-stranded RNA-specific endoribonuclease
  • dicer 1, ribonuclease type III
  • Dicer
  • Dicer1, Dcr-1 homolog (Drosophila)
  • EC 3.1.26
  • EC 3.1.26.-
  • EC 3.1.26.3
  • Helicase MOI
  • Helicase with RNase motif
  • helicase-moi
  • HERNADicer1, Dcr-1 homolog
  • K12H4.8-LIKE
  • KIAA0928endoribonuclease Dicer

Background

Dicer is an RNaseIII-like enzyme which cleaves double-stranded RNA into 21-23 nt RNAs. These RNAs are referred to as siRNA (short interfering RNA). It is believed to be essential in siRNA-mediated gene suppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dicer Antibody (NB200-591)(13)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Human,Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(2)
WB
(6)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Rat
(1)
Human,Mouse
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB200-591 Applications Species
de Almeida DC, Bassi EJ, Azevedo H et al. A Regulatory miRNA-mRNA Network Is Associated with Tissue Repair Induced by Mesenchymal Stromal Cells in Acute Kidney Injury Front Immunol. Jan 18 2016 [PMID: 28096802] (WB, IHC, Mouse) WB, IHC Mouse
Lu XF, Zhou YJ, Zhang L et al. Loss of Dicer1 impairs hepatocyte survival and leads to chronic inflammation and progenitor cell activation World J. Gastroenterol. 2015 Jun 07 [PMID: 26074697] (IHC-P, Mouse) IHC-P Mouse
Chiappinelli KB, Haynes BC, Brent MR, Goodfellow PJ. Reduced DICER1 Elicits an Interferon Response in Endometrial Cancer Cells Mol Cancer Res 2012 Feb 21 [PMID: 22252463] (WB, Human) WB Human
Grelier G, Voirin N, Ay AS et al. Prognostic value of Dicer expression in human breast cancers and association with the mesenchymal phenotype. Br J Cancer. 2009 Aug 18 [PMID: 19672267] (WB, IHC-P)

Details:
1. WB: Four human breast cancer cell lines; HMEC, HMEC+hTERT, HMEC+LT+hTERT, and HMLER, Fig 2A. 2. WB: Five mouse mammary cell lines; 67NR, 168FARN, 4TO7, 66c14, and 4T1, Fig 3A. 3. IHC (Paraffin): Normal breast and ductal carcinoma tissue, Fig 4A, 4B, &
WB, IHC-P
Chen JF, Murchison EP, Tang R et al. Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure. Proc Natl Acad Sci U S A. 2008 Feb 12 [PMID: 18256189]
Poleshko A, Palagin I, Zhang R et al. Identification of cellular proteins that maintain retroviral epigenetic silencing: evidence for an antiviral response. J Virol. 2008 Mar [PMID: 18094192] (WB)

Details:
WB: HeLa TI-C cells treated with Dicer siRNA, Fig 3C. Note: The (IMG-538) Dicer pAb was siRNA validated by WB in HeLa TI-C cells, Fig 3C.
WB
Barbato C, Ciotti MT, Serafino A et al. Dicer expression and localization in post-mitotic neurons. Brain Res. 2007 Oct 17 [PMID: 17888888] (WB, Rat)

Details:
Rat neuronal primary cultures from 8 day old Wistar rats. IF (Figs 1, 2B, 2C, 3A, 4-8); WB (Figs 2A, 7A). Notes: 1. Dicer was detected as a single band just below the 220 kDa marker in total cell lysates from hippocampal, cortical, and cerebellar granule
WB Rat
Grivna ST, Pyhtila B, Lin H. MIWI associates with translational machinery and PIWI-interacting RNAs (piRNAs) in regulating spermatogenesis. Proc Natl Acad Sci U S A. 2006 Sep 5 [PMID: 16938833]
Lugli G, Larson J, Martone ME et al. Dicer and eIF2c are enriched at postsynaptic densities in adult mouse brain and are modified by neuronal activity in a calpain-dependent manner. J Neurochem. 2005 Aug [PMID: 16092937] (WB, Human,Mouse)

Details:
WB: Recombinant human dicer and a cytosolic fraction of adult mouse brain (data not shown). 1. Recombinant dicer was recognized at ~220 and 125 kDa bands. The 220 kDa band represents full length dicer and the 125 kDa a fragmented form of dicer. 2. Mouse b
WB Human,Mouse
Doi N et al. Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer eIF2C translation initiation factors. Curr Biol 13:41-6. 2003 [PMID: 12526743]
Show All 13 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Dicer Antibody (NB200-591). (Showing 1 - 1 of 1 FAQs).

  1. Does this antibody work for immunofluorescence staining in mouse tissue?
    • This antibody lists both Mouse and IF. We guarantee all of our products to work in all species and applications listed.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Dicer Antibody (NB200-591)

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Research Areas for Dicer Antibody (NB200-591)

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Blogs on Dicer.

Slicing and Dicing RNA with Dicer
Dicer is an RNaseIII-like enzyme capable of cleaving double-stranded RNA (dsRNA) into smaller 21-23 nt RNA fragments known as short interfering RNA (siRNAs). It targets the selective degradation of complementary RNAs in a posttranscriptional gene sile...  Read full blog post.

Ago2 antibodies and dicer-independent biogenesis of miRNA
Ago2, also called eIF2C2, antibody is one of 37 reagents targeted to the Argonaute protein family that we at Novus Biologicals have in our antibody catalogue. Argonaute proteins are encoded by genes which play an important role in regulating the contr...  Read full blog post.

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Bioinformatics

Gene Symbol DICER1
Entrez
Uniprot