Dicer Antibody (13D6)

Product Discontinued

Dicer Antibody (13D6) Summary

• Immunogen: Synthetic peptide to the N-terminus of the human protein.
• Specificity: Dicer (13D6)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DICER1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/ Immunofluorescence Assay Dependent
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:100-1:2000
• Application Notes: This antibody is useful for Western blot and immunofluorescence. Suggested WB dilution assumes that sample in 1x BLOTTO or 3% BSA in PBS (with ECL or ECL plus).
• Theoretical MW: 217 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, Mouse Rat and Lizard Dicer. Other species have not been tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Dicer Antibody (13D6)

• DCR1
• dicer 1, double-stranded RNA-specific endoribonuclease
• dicer 1, ribonuclease type III
• Dicer
• Dicer1, Dcr-1 homolog (Drosophila)
• EC 3.1.26
• EC 3.1.26.-
• EC 3.1.26.3
• Helicase MOI
• Helicase with RNase motif
• helicase-moi
• HERNADicer1, Dcr-1 homolog
• K12H4.8-LIKE
• KIAA0928endoribonuclease Dicer

Background

RNA interference is an evolutionarily conserved gene silencing pathway in which the endonuclease, Dicer cleaves double stranded RNA into small interfering RNAs. Dicer is a multidomain protein related to the RNase III protein family. Dicer is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small interfering RNA (siRNA) component that represses gene expression. Dicer related RNA interference machinery is also involved in the formation of the heterochromatin structure in organisms such as yeast and higher vertebrate cells. In mammalian cells, both microRNAs (miRNAs) and small interfering RNAs (siRNAs) are thought to be loaded into the same RNA induced silencing complex (RISC), where they guide mRNA degradation or translation silencing depending on the complementarity of the target. Two transcript variants encoding the same protein have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DICER1
• Entrez:
  • 23405(Human)
  • 192119(Mouse)
  • 299284(Rat)
• Uniprot:
  • Q9UPY3(Human)