![Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody NB100-586 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/DHX15-Antibody-Western-Blot-NB100-586-img0006.jpg "Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX15 antibody NB100-586 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
| Reactivity | Hu, Mu, Pm-OrSpecies Glossary |
| Applications | WB, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit DHX15 Antibody - BSA Free (NB100-586) is a polyclonal antibody validated for use in WB and IP. Anti-DHX15 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 795) of human DEAH box polypeptide 15 using the numbering given in Swiss-Prot entry O43143 (GeneID 1665). |
| Specificity | NB100-586 is specific for human DHX 15 protein. |
| Predicted Species | Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DHX15 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
||
| Application Notes | Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:5,000 - 1:15,000 Immunoprecipitation: 1 to 5 ug/mg lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated. |
||
| Control |
|
||
| Reviewed Applications |
|
||
| Publications |
|
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
WB | Human | 06/19/2025 |
Summary
|
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 on HeLa whole cell lysate using NB100-586. Immunoprecipitated DHX15 was blotted using NB100-585 at 0.1 mcg/ml. NB100-585 was also used unsuccessfully for IP.](http://images.novusbio.com/fullsize/DHX15-Antibody-Western-Blot-NB100-586-img0002.jpg "Western Blot: DHX15 Antibody [NB100-586] - Detection of Human DHX15 on HeLa whole cell lysate using NB100-586. Immunoprecipitated DHX15 was blotted using NB100-585 at 0.1 mcg/ml. NB100-585 was also used unsuccessfully for IP.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
