![Western Blot: DHX15 Antibody [NB100-585] - Detection of Human DHX15 on HeLa whole cell lysate using NB100-585. Successful IP of DHX15 was accomplished using NB100-586 at 1 mcg/mg lysate.](http://images.novusbio.com/fullsize/DHX15-Antibody-Western-Blot-NB100-585-img0001.jpg "Western Blot: DHX15 Antibody [NB100-585] - Detection of Human DHX15 on HeLa whole cell lysate using NB100-585. Successful IP of DHX15 was accomplished using NB100-586 at 1 mcg/mg lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit DHX15 Antibody - BSA Free (NB100-585) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Maps to a region between residues 350 and 400 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 15 using the numbering given in Swiss-Prot entry O43143 (GeneID 1665). |
| Specificity | Reactivity with mouse and X. laevis DHX15 is likely owing to conservation of sequence between these orthologs and human DHX15 at the site to which the epitope maps. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DHX15 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is useful in Western Blot. Not recommended for Immunoprecipitation. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: DHX15 Antibody [NB100-585] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DHX15 used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/DHX15-Antibody-Immunohistochemistry-NB100-585-img0004.jpg "Immunohistochemistry: DHX15 Antibody [NB100-585] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DHX15 used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")