DHX15 Antibody

Images

 
Western Blot: DHX15 Antibody [NB100-585] - Detection of Human DHX15 on HeLa whole cell lysate using NB100-585. Successful IP of DHX15 was accomplished using NB100-586 at 1 mcg/mg lysate.
Immunohistochemistry: DHX15 Antibody [NB100-585] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DHX15 used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

DHX15 Antibody Summary

Immunogen
Maps to a region between residues 350 and 400 of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 15 using the numbering given in Swiss-Prot entry O43143 (GeneID 1665).
Specificity
Reactivity with mouse and X. laevis DHX15 is likely owing to conservation of sequence between these orthologs and human DHX15 at the site to which the epitope maps.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHX15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody is useful in Western Blot. Not recommended for Immunoprecipitation.
Positive Control
DHX15 Lysate (NBL1-09876)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DHX15 Antibody

  • ATP-dependent RNA helicase #46
  • DBP1DEAH box protein 15
  • DDX15putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 15
  • DEAH (Asp-Glu-Ala-His) box polypeptide 15
  • EC 3.6.1
  • EC 3.6.4.13
  • HRH2DEAD/H box-15
  • PRP43
  • PRPF43
  • PrPp43p
  • RNA helicase 2

Background

Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA. The protein encoded by this gene is a putative ATP-dependent RNA helicase implicated in pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DHX15
Entrez
Uniprot