DGK-gamma Antibody (613531)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, C2C12 mouse myoblast cell line, C6 rat glioma cell line, and SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat DGK- gamma Monoclonal Antibody (Catalog # MAB6389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK- gamma at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 613531
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DGK-gamma Antibody (613531) Summary

• Immunogen: E. coli-derived recombinant human DGK‑ gamma
  Gly2-Ser126
  Accession # P49619
• Specificity: Detects human, mouse, and rat DGK-gamma in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DGKG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DGK-gamma Antibody (613531)

• DAG kinase gamma
• DAGK3
• DAGK3MGC104993
• DGKG
• DGKgamma
• DGK-gamma
• diacylglycerol kinase gamma
• diacylglycerol kinase, gamma (90kD)
• diacylglycerol kinase, gamma 90kDa
• diacylglyerol kinase gamma
• Diglyceride kinase gamma
• EC 2.7.1.107
• MGC133330

Background

DGK-gamma (Diacylglycerol kinase gamma) is a 90 kDa, 791 amino acid (aa), type I member of the eukaryocytic diacylglycerol kinase family of enzymes. DGK-gamma possesses EF-hand, C1/Cys-rich zinc finger and catalytic domains, and is highly enriched in the retina and in cerebellar Purkinje cells. Expression elsewhere is limited to inactive isoforms missing kinase domain aa 451-475, or zinc finger aa 333-372. Upon calcium and PKC gamma -mediated activation, it translocates to the plasma membrane. DGK-gamma is also found in the nucleus, where it may promote cell cycle progression. Over aa 1-126, human DGK-gamma shares 83% and 79% aa identity with mouse and rat DGK-gamma , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGKG
• Uniprot:
  • P49619(Human)