DGK-gamma Antibody (613531) [Alexa Fluor® 647]

• Applications: WB
• Clone: 613531
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

DGK-gamma Antibody (613531) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human DGK‑γ
  Gly2-Ser126
  Accession # P49619
• Specificity: Detects human, mouse, and rat DGK‑ gamma in Western blots.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

DGK-gamma (Diacylglycerol kinase gamma) is a 90 kDa, 791 amino acid (aa), type I member of the eukaryocytic diacylglycerol kinase family of enzymes. DGK-gamma possesses EF-hand, C1/Cys-rich zinc finger and catalytic domains, and is highly enriched in the retina and in cerebellar Purkinje cells. Expression elsewhere is limited to inactive isoforms missing kinase domain aa 451‑475, or zinc finger aa 333‑372. Upon calcium and PKC gamma -mediated activation, it translocates to the plasma membrane. DGK-gamma is also found in the nucleus, where it may promote cell cycle progression. Over aa 1‑126, human DGK-gamma shares 83% and 79% aa identity with mouse and rat DGK-gamma , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.