CHST8 Antibody

Immunohistochemistry-Paraffin: CHST8 Antibody [NBP1-83396] - Staining of human prostate shows membranous and cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: CHST8 Antibody [NBP1-83396] - Staining of human pituitary gland shows strong cytoplasmic positivity in endocrine cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CHST8 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QVPGIKFNIRPRQPHHDLPPGGSQDGDLKEPTERVTRDLSSGAPRGRNLPAPDQPQPPLQRGTRLRLRQRRRRLLIKKMPAAATIPANSSDAPFIRPGPGTLDGRWVSLHRSQQE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHST8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB, ICC/IF reported in scientific literature (PMID: 22289416). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  CHST8 Protein (NBP1-83396PEP)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22289416).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CHST8 Antibody

• carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8
• carbohydrate sulfotransferase 8
• EC 2.8.2.-
• GalNAc-4-O-sulfotransferase 1
• GalNAc4ST
• GALNAC4ST1
• GALNAC-4-ST1
• galNAc4ST-1
• N-acetylgalactosamine-4-O-sulfotransferase 1

Background

Sulfate groups in carbohydrates confer highly specific functions on glycoproteins, glycolipids, and proteoglycans and are critical for cell-cell interaction, signal transduction, and embryonic development. Sulfotransferases, such as CHST8, carry out sulfation of carbohydrates (Hiraoka et al., 2001 [PubMed 11445554]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHST8 Antibody (NBP1-83396)(2)

Publications using NBP1-83396

• Qundos U, Hong MG, Tybring G et al. Profiling post-centrifugation delay of serum and plasma with antibody bead arrays. J Proteomics 2013-12-16 [PMID: 23631827]
• Cabral RM, Kurban M, Wajid M et al. Whole-exome sequencing in a single proband reveals a mutation in the CHST8 gene in autosomal recessive peeling skin syndrome. Genomics 2012-04-01 [PMID: 22289416] (WB, ICC/IF, Human)

Bioinformatics

• Gene Symbol: CHST8