DGK-delta Antibody (625423) [Alexa Fluor® 750]

• Applications: WB
• Clone: 625423
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

DGK-delta Antibody (625423) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human DGF-δ
  Ile570-Thr717
  Accession # Q16760
• Specificity: Detects human DGF-δ in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Diacylglycerol kinase delta (DGK-δ) is a widely expressed 140 kDa member of the DGK family of kinases that phosphorylate the lipid diacylglycerol. DGK-δ contains a pleckstrin homology domain (aa 53-146), two zinc finger domains (aa 163-213 and aa 235-286), a catalytic domain (aa 317-451), and a sterile alpha motif (aa 1145‑1208). An isoform of DGK-δ with a shortened N-terminus is expressed predominantly in ovary and spleen. The two isoforms can form intracellular homooligomers or heterooligomers with each other. The oligomers are disrupted by PKC following phorbol ester stimulation. DGK-δ regulates PKC mediated signaling downstream of the EGF receptor. Within aa 570-717, human DGK-δ shares 82% aa sequence identity with mouse and rat DGK-δ.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.