DET1 Antibody - BSA Free

Western Blot: DET1 Antibody [NBP2-92595] - Western blot analysis of extracts of various cell lines, using DET1 antibody (NBP2-92595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DET1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DET1 Antibody - BSA Free (NBP2-92595) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 15-240 of human DET1 (NP_060466.2). HVSTIKPRRIQNQNVIHRLERRRISSGKAGTHWHQVRVFHQNVFPNFTVVNVEKPPCFLRKFSPDGRYFIAFSSDQTSLEIYEYQGCQAAEDLLQGYEGEILSNGNDQRSVNIRGRLFERFFVLLHITNVAANGEHLNRECSLFTDDCRCVIVGSAAYLPDEPHPPFFEVYRNSESVTPNPRSPLEDYSLHIIDLHTGRLCDTRTFKCDKVVLSHNQGLYLYKNIL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DET1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DET1 Antibody - BSA Free

• de-etiolated 1
• de-etiolated homolog 1 (Arabidopsis)
• De-etiolated-1 homolog
• DET1 homolog
• FLJ10103
• MGC126156
• MGC126157

Background

DET1, also known as DET1 homolog, consists of a 63.8 kDa and a 65.1 kDa isoform and is involved in the ubiquitination of target proteins as well as their consequent degradation. This protein is found in large quantities in the ovary and in small quantities in some lymphoid organs. There is currently no research with this protein and its effect on diseases. In antigen processing, immune system, and upiquitin mediated proteolysis pathways, the protein interacts with DDA1, DDB2, RFWD2, RBX1, and CUL4A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DET1