Desmoplakin Antibody - BSA Free

Immunohistochemistry;Desmoplakin Antibody[NBP3-29477]Detection of human Desmoplakin by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Desmoplakin used at a dilution of 1:1,000 . Detection: DAB

Immunoprecipitation:Desmoplakin AntibodyNBP3-29478] -Detection of human Desmoplakin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Desmoplakin antibody NBP3-29478 used for IP at 6 µg/mg lysate. Desmoplakin was also immunoprecipitated by rabbit anti-Desmoplakin., which recognizes an upstream epitope. For blotting immunoprecipitated Desmoplakin, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Desmoplakin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Desmoplakin Antibody - BSA Free (NBP3-29478) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: between 1900 and 1950
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DSP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Desmoplakin Antibody - BSA Free

• 250/210 kDa paraneoplastic pemphigus antigen
• DCWHKTA
• desmoplakin (DPI, DPII)
• desmoplakin I
• desmoplakin II
• Desmoplakin
• DP
• DPI
• DPII
• DSP
• KPPS2
• PPKS2

Background

Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DSP