Immunohistochemistry-Paraffin: Desmocollin-1 Antibody [NBP1-88099] - Staining of human skin shows moderate to strong membranous positivity in epidermal cells.
Immunohistochemistry-Paraffin: Desmocollin-1 Antibody [NBP1-88099] - Staining of human fallopian tube shows very weak membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Desmocollin-1 Antibody [NBP1-88099] - Staining of human prostate shows no membranous positivity in glandular cells.
Simple Western: Desmocollin-1 Antibody [NBP1-88099] - Simple Western lane view shows a specific band for Desmocollin-1 in 0.5 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing ...read more
Simple Western: Desmocollin-1 Antibody [NBP1-88099] - Electropherogram image(s) of corresponding Simple Western lane view. Desmocollin-1 antibody was used at 1:60 dilution on RT-4 and U-251MG lysate(s).
This antibody was developed against Recombinant Protein corresponding to amino acids: SCTGTLVVHLDDYNDHAPQIDKEVTICQNNEDFAVLKPVDPDGPENGPPFQFFLDNSASKNWNIEEKDGKTAILRQRQNLDYNYYSVPIQIKDRHGLVATHMLTVRVCDCSTPSECRMKDKSTR
Specificity of human Desmocollin-1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
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For IHC-Paraffin, HIER pH 6 retrieval is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Use in Western blot reported in scientific literature (PMID: 31158401).
Paraffin sections of canine folicular skin (dogs with pemphigus foliaceus)
Background Pemphigus foliaceus is the most common autoimmune disease in dogs. It is characterized histologically by subcorneal pustules and acantholysis. Desmocollin-1 is the major target antigen of autoantibodies in dogs. Previous immunostaining studies have shown possible pathogenic similarities between canine and human pemphigus.
Objetives To investigate the pathogenesis of acantholysis in canine Pemphigus Foliaceus, we decided to review the main histopathological findings and observe the variations of the labeling of desmocollin-1 by immunohistochemistry in skin biopsies from sick dogs.
Material and methods Five cases of dogs with Pemphigus Foliaceus were selected and histopathological findings were reviewed by hematoxylin-eosin staining. Then the samples were stained following an immunohistochemical protocol, with an anti-desmocolin-1 antibody.
Results The 5 cases presented typical histological characteristics of PFc: pustules in the superficial layers of the epidermis, acantholytic keratinocytes and neutrophilic exocytosis. We have validated the use of the anti-dsc-1 antibody in dogs, for immunhistochemistry in skin biopsies preserved in paraffin. We have observed that, in dogs with PF, the immunohistochemical pattern of desmocollin-1 was altered.
Conclusions Staining of desmocollin-1 is altered in dogs with PF. We were able to corroborate the pathogenic mechanisms already demonstrated by immunohistochemistry. Thus, immunohistochemical staining for this protein may be useful in diagnosis. Its use, to study precise mechanisms, is limited and more biotechnologic tools are needed to complement the knowledge on this disease.
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