Desmocollin-1 Antibody (2906A) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: IHC
• Clone: 2906A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

Desmocollin-1 Antibody (2906A) [Alexa Fluor® 532] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line, NS0-derived human Desmocollin-1
  Arg135-Asn686
  Accession # Q08554
• Specificity: Detects human Desmocollin-1 in direct ELISA.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmocollin-1 Antibody (2906A) [Alexa Fluor® 532]

• cadherin family member 1
• CDHF1
• desmocollin 1
• Desmocollin1
• Desmocollin-1
• desmosomal glycoprotein 2/3
• DG2/DG3
• DG2/DG3
• DSC1

Background

Desmocollin-1 (DSC1) is a 100 kDa-110 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules (1-3). Human DSC1 is synthesized with a 105 amino acid (aa) propeptide. The mature protein consists of a 557 aa extracellular domain (ECD) that contains five cadherin-like domains, a 23 aa transmembrane segment, and a 180 aa cytoplasmic domain (4, 5). Within the ECD, human DSC1 shares 79% and 82% aa sequence identity with bovine and mouse DSC1, respectively, and 53% with human DSC2 and DSC3. An alternately spliced isoform has a substituted and truncated cytoplasmic domain (5). DSC1 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is predominantly expressed in the outer layers of stratified epithelia of the skin, tongue, and hair follicle root sheath (6, 7). It is required for both promoting epidermal differentiation and maintaining epidermal strength (8). DSC2 and DSC3, by contrast, are preferentially localized in the basal and suprabasal layers of the epidermis (1). DSC1 is not expressed in normal colon epithelium, but it is induced during colon carcinogenesis (9). Loss of DSC1 function in the skin disorders IgA pemphigus and Netherton Syndrome results from autoimmune targeting or enhanced KLK-7 mediated proteolysis, respectively (10-12).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.