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Desert Hedgehog/Dhh Antibody

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Western Blot: Desert Hedgehog/Dhh Antibody [NBP2-42834] - Analysis of 30 ug whole cell lysate. A: A431 10% SDS PAGE diluted at 1:10000.
Western Blot: Desert Hedgehog/Dhh Antibody [NBP2-42834] - Analysis of 30 ug whole cell lysate. A: A431 10% SDS PAGE diluted at 1:10000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Desert Hedgehog/Dhh Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Dhh. The exact sequence is proprietary.
Predicted Species
Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1:5000-1:20000
Application Notes
IHC-FR-Assay dependent
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for Desert Hedgehog/Dhh Antibody

  • desert hedgehog (Drosophila) homolog
  • desert hedgehog homolog
  • desert hedgehog protein
  • Desert Hedgehog
  • DHH
  • HHG-3MGC35145

Background

Dhh encodes a member of the Hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog #1845-SH/CF) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1‑0.4 μg/mL.
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Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Patched 1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Patched 1/PTCH Antibody (5c7) [NBP1-47945] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Patched 1 mouse monoclonal antibody.
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Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Staining in canine hair follicle. Image from a verified customer review.
Knockdown Validated: GLI-1 Antibody - BSA Free [NB600-600] - GLI-1 Antibody [NB600-600] - Inhibition of Hh signaling in CAFs blocks its effects on lymphangiogenesis in vivo and in vitro. (E) IHC staining for Ki-67, -SMA, Gli-1 and LYVE1 in tumour sections from mice in the two groups. Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31012844/) licensed under a CC-BY licence.
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Western Blot: HFE Antibody (1G12) [H00003077-M01] - Analysis of HFE expression in transfected 293T cell line by HFE monoclonal antibody (M01), clone 1G12.Lane 1: HFE transfected lysate(40.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: HFE Antibody (1G12) [H00003077-M01] - Analysis of monoclonal antibody to HFE on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: HFE Antibody (1G12) [H00003077-M01] - HFE monoclonal antibody (M01), clone 1G12. Analysis of HFE expression in A-431.
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12.5% SDS-PAGE Stained with Coomassie Blue.
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NBP2-88921
Immunocytochemistry/Immunofluorescence: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) [NBP2-88921] - Staining of U-2 OS cells using the Anti-NR5A1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody (CL9101) [NBP2-88921] - Staining of human breast shows weak to moderate nuclear positivity in glandular cells.
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Western Blot: Smoothened Antibody [NBP3-06699] - Western blot analysis of lysates from HUVEC cells, using Smoothened Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry-Paraffin: Smoothened Antibody [NBP3-06699] - Human Small Intestine, Plasma: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry-Paraffin: Smoothened Antibody [NBP3-06699] - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
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Western Blot: SMOX Antibody [NBP3-04509] - Western blot analysis of extracts of various cell lines, using SMOX antibody (NBP3-04509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
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Applications: IHC, IHC-P, PEP-ELISA, WB
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ELISA: SRY Antibody (1G4) [NBP2-37463] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Western Blot: SRY Antibody (1G4) [NBP2-37463] - Analysis using SRY mAb against human SRY recombinant protein. (Expected MW is 49.4 kDa)
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Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
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Western Blot: Splicing Factor 1 Antibody (2E12) [H00007536-M01] - Analysis of SF1 expression in transfected 293T cell line by SF1 monoclonal antibody (M01), clone 2E12.Lane 1: SF1 transfected lysate (Predicted MW: 59.7 KDa).Lane 2: Non-transfected lysate.
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Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3690) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for GLI‑3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, KATO‑III human gastric carcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human SOX9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3075) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX9 at approximately 75 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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PP-H7431-00
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[Unconjugated]
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NBP1-33581
Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581] - Mouse pancreas. Factor IX antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581] - Mouse liver. Factor IX stained by Factor IX antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunocytochemistry/Immunofluorescence: Factor IX Antibody [NBP1-33581] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX protein stained by Factor IX antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Bioinformatics

Gene Symbol DHH