Dermokine beta Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

Dermokine beta Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human DMKN.
  
  

  Source: E. coli

  Amino Acid Sequence: GGNGHKPGNSETSPGMFNFDTFWKNFKSKLGFINWDAINKNQVPPPSTRALLYFSRLWEDFKQNTPFLNWKAIIEGADASSLQKRAGRADQNYNYNQHAYPTAYGGKYSVKTPAKGGVSPSSSASR

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: DMKN
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-86840.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Dermokine beta Recombinant Protein Antigen

• dermokine
• Epidermis-specific secreted protein SK30/SK89
• FLJ79374
• UNQ729
• ZD52F10

Background

This gene is upregulated in inflammatory diseases, and it was first observed as expressed in the differentiated layers of skin. The most interesting aspect of this gene is the differential use of promoters and terminators to generate isoforms with unique cellular distributions and domain compliments. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, but the full-length nature of some of them has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Dermokine beta Recombinant Protein Antigen (NBP1-86840PEP)(2)

Publications using NBP1-86840PEP

• Xinhui Wu, I. Sophie T. Bos, Thomas M. Conlon, Meshal Ansari, Vicky Verschut, Luke van der Koog, Lars A. Verkleij, Angela D’Ambrosi, Aleksey Matveyenko, Herbert B. Schiller, Melanie Königshoff, Martina Schmidt, Loes E. M. Kistemaker, Ali Önder Yildirim, Reinoud Gosens A transcriptomics-guided drug target discovery strategy identifies receptor ligands for lung regeneration Science Advances 2022-03-01 [PMID: 35319981]
• Wu X Regenerative Pharmacology for COPD Thorax 2019-04-04 [PMID: 30940772]

Bioinformatics

• Gene Symbol: DMKN