Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-49674] - Analysis of DEPDC6 in A. human liver and B. mouse liver.
Immunocytochemistry/ Immunofluorescence: DEPTOR/DEPDC6 Antibody [NBP1-49674] - Antibody was tested in MCF7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-49674] - Staining of DEPTOR in mouse spleen using DAB with a hematoxylin counterstain.
This DEPTOR antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. In Western Blot, a band is seen at ~46 kDa. In ICC/IF, cytoplasmic staining was observed in serum starved HeLa cells. In IHC-P, staining was observed in the cytoplasm of mouse spleen. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Human and mouse. Predicted to react with bovine based on 100% sequence homology.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Manufactured by Genomic Antibody Technology™. GAT FAQs
Alternate Names for DEPTOR/DEPDC6 Antibody
DEP domain containing 6
DEP domain containing mTOR interacting protein
DEP domain containing MTOR-interacting protein
DEP domain-containing mTOR-interacting protein
DEP domain-containing protein 6
DEPTOR (DEP domain-containing mTOR-interacting protein), also called DEP domain-containing protein 6 (DEPDC6), is a part of mTORC1 (MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR) and mTORC2 (MTOR, MLST8, PROTOR1, RICTOR, MAPKAP1 and DEPTOR) complexes. DEPTOR functions as a negative regulator of the mTORC1/mTORC2 signaling pathways, leading to inhibition of activity of both kinase complexes. DEPTOR phosphorylation can weaken the interaction with MTOR within mTORC1 and mTORC2 complexes. DEPTOR has been shown to act as a tumor suppressor via mTORC1/mTORC2 blocking to inhibit protein synthesis as well as AKT's proliferation and survival related signaling. However, in certain conditions, DEPTOR behaves as an oncogene by relieving the feedback inhibition from S6K1 to PI3K, thus causing AKT activation. The stability of DEPTOR is controlled by SCFbeta-TrCP E3 ubiquitin ligase, and S6K1 and RSK1 are kinases that phosphorylate DEPTOR for facilitating its binding to betaTrCP for subsequent degradation. DEPTOR is down-regulated in a number of malignancies, however, is overexpressed in a subset of multiple myelomas harbouring cyclin D1/D3 or c-MAF/MAFB translocations.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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