DEPTOR/DEPDC6 Antibody

Images

 
Western Blot: DEPTOR/DEPDC6 Antibody [NBP1-49674] - Analysis of DEPDC6 in A. human liver and B. mouse liver.
Immunocytochemistry/ Immunofluorescence: DEPTOR/DEPDC6 Antibody [NBP1-49674] - Antibody was tested in MCF7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
Immunohistochemistry: DEPTOR/DEPDC6 Antibody [NBP1-49674] - Staining of DEPTOR in mouse spleen using DAB with a hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

DEPTOR/DEPDC6 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human DEPTOR protein (within residues 10-200). [Swiss-Prot Q8TB45]
Localization
Intracellular.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DEPTOR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This DEPTOR antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. In Western Blot, a band is seen at ~46 kDa. In ICC/IF, cytoplasmic staining was observed in serum starved HeLa cells. In IHC-P, staining was observed in the cytoplasm of mouse spleen. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Positive Control
Liver Lysate (NB820-59233)
Liver Lysate (NB820-59662)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-49674 in the following applications:

Publications
Read Publications using
NBP1-49674 in the following applications:

Reactivity Notes

Human and mouse. Predicted to react with bovine based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for DEPTOR/DEPDC6 Antibody

  • DEP domain containing 6
  • DEP domain containing mTOR interacting protein
  • DEP domain containing MTOR-interacting protein
  • DEP domain-containing mTOR-interacting protein
  • DEP domain-containing protein 6
  • DEP.6
  • DEPDC6
  • DEPDC6FLJ12341
  • DEPTOR
  • DKFZp564B1778
  • FLJ12428
  • FLJ13854

Background

DEPTOR (DEP domain-containing mTOR-interacting protein), also called DEP domain-containing protein 6 (DEPDC6), is a part of mTORC1 (MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR) and mTORC2 (MTOR, MLST8, PROTOR1, RICTOR, MAPKAP1 and DEPTOR) complexes. DEPTOR functions as a negative regulator of the mTORC1/mTORC2 signaling pathways, leading to inhibition of activity of both kinase complexes. DEPTOR phosphorylation can weaken the interaction with MTOR within mTORC1 and mTORC2 complexes. DEPTOR has been shown to act as a tumor suppressor via mTORC1/mTORC2 blocking to inhibit protein synthesis as well as AKT's proliferation and survival related signaling. However, in certain conditions, DEPTOR behaves as an oncogene by relieving the feedback inhibition from S6K1 to PI3K, thus causing AKT activation. The stability of DEPTOR is controlled by SCFbeta-TrCP E3 ubiquitin ligase, and S6K1 and RSK1 are kinases that phosphorylate DEPTOR for facilitating its binding to betaTrCP for subsequent degradation. DEPTOR is down-regulated in a number of malignancies, however, is overexpressed in a subset of multiple myelomas harbouring cyclin D1/D3 or c-MAF/MAFB translocations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DEPTOR/DEPDC6 Antibody (NBP1-49674)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(8)
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Human
(3)
Mouse
(3)
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Showing Publications 1 - 9 of 9.
Publications using NBP1-49674 Applications Species
Caron A, Mouchiroud M, Gautier N et al. Loss of hepatic DEPTOR alters the metabolic transition to fasting. Mol Metab. Feb 17 2017 [PMID: 28462079] (WB, Mouse) WB Mouse
Song HI, Yoon MS. PLD1 regulates adipogenic differentiation through mTOR - IRS-1 phosphorylation at serine 636/639. Sci Rep. Nov 22 2016 [PMID: 27872488] (WB, Mouse) WB Mouse
Carbonneau M, M Gagne L, Lalonde ME et al. The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. Nat Commun 2016 Sep 14 [PMID: 27624942] (WB) WB
Caron A, Labbe SM, Lanfray D et al. Mediobasal hypothalamic overexpression of DEPTOR protects against high-fat diet-induced obesity. Mol Metab 2016 Feb [PMID: 26909318] (WB) WB
Caron A, Baraboi ED, Laplante M et al. DEP domain-containing mTOR-interacting protein in the rat brain: distribution of expression and potential implication J. Comp. Neurol. 2014 Aug 27 [PMID: 25159114]
Brundage ME, Tandon P, Eaves DW et al. MAF mediates crosstalk between Ras-MAPK and mTOR signaling in NF1. Oncogene 2014 Feb 10 [PMID: 24509877] (WB, ICC/IF, Human) WB, ICC/IF Human
Fernandez-Saiz V, Targosz BS, Lemeer S et al. SCF(Fbxo9) and CK2 direct the cellular response to growth factor withdrawal via Tel2/Tti1 degradation and promote survival in multiple myeloma Nat Cell Biol 2012 Dec 23 [PMID: 23263282] (WB, Human) WB Human
Jegg AM, Ward TM, Iorns E et al. PI3K independent activation of mTORC1 as a target in lapatinib-resistant ERBB2+ breast cancer cells Breast Cancer Res Treat 2012 Oct 23 [PMID: 23089982] (WB, Human) WB Human
Caron A, Labbe S, LanfrayD Mediobasal Hypothalamic Overexpression of Deptor Protects Against High-Fat Diet-Induced Obesity Molecular Metabolism 2015 Nov 2 (WB, Mouse) WB Mouse

Review for DEPTOR/DEPDC6 Antibody (NBP1-49674) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 application: WB.

Reviews using NBP1-49674:
Filter by Applications
WB
(1)
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Images Ratings Applications Species Date Details
Western Blot DEPTOR/DEPDC6 NBP1-49674
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reviewed by:
Anonymous
WB 08/29/2014
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Summary

ApplicationWestern Blot
LotA-2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DEPTOR/DEPDC6 Antibody (NBP1-49674) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49674AF405
Alexa Fluor 488 Labeled NBP1-49674AF488
Alexa Fluor 647 Labeled NBP1-49674AF647
Alexa Fluor 700 Labeled NBP1-49674AF700
Biotin Labeled NBP1-49674B
DyLight 405 Labeled NBP1-49674V
DyLight 488 Labeled NBP1-49674G
DyLight 550 Labeled NBP1-49674R
DyLight 650 Labeled NBP1-49674C
FITC Labeled NBP1-49674F
HRP Labeled NBP1-49674H

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
08/29/2014
Application: WB
Species:

Bioinformatics

Gene Symbol DEPTOR
Entrez
Uniprot