Dectin-2/CLEC6A alpha Isoform Antibody [Alexa Fluor™ Plus 555]

• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 555

Dectin-2/CLEC6A alpha Isoform Antibody [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects mouse Dectin‑2/CLEC6A alpha Isoform in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human DLEC is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dectin-2/CLEC6A alpha Isoform Antibody [Alexa Fluor™ Plus 555]

• Dectin-2/CLEC6A alpha Isoform

Background

Dectin-2 alpha , also known as CLEC6A, CLECSF10, and NKCL, belongs to the C-type lectin family of transmembrane immune regulatory glycoproteins. Dectin-2 alpha , CLEC4A/DCIR, CLEC4B/DCAR, CLEC4C/DLEC, CLEC4D/MCL, and CLEC4E/mincle constitute a subgroup of these molecules that exhibit approximately 40% amino acid (aa) sequence identity in their extracellular domains (ECD) and have a conserved cysteine spacing in their carbohydrate recognition domains (CRD) (1, 2). Mature mouse Dectin-2 alpha consists of a short cytoplasmic tail, a transmembrane segment, and an ECD with a stalk region and one CRD (3, 4). Alternate splicing leads to partial deletion of the transmembrane segment and stalk ( beta isoform) or a portion of the CRD ( gamma isoform) (4). The full length Dectin-2 alpha isoform is a 27 kDa molecule that is primarily expressed on the surface of tissue macrophages and their precursors (3‑6). The CRD of Dectin-2 alpha contains an EPN motif which is characteristic of calcium-dependent mannose-binding lectins. Dectin-2 alpha selectively interacts with high mannose structures in the Man₉GlcNAc₂ configuration (7). It mediates the recognition of a variety of microorganisms, particularly the filamentous forms of yeast and fungii (7, 8). The short cytoplasmic tail does not contain signaling motifs but mediates association with the ITAM-containing Fc receptor gamma subunit in macrophages (8). Ligation of Dectin-2 alpha induces tyrosine phosphorylation of the gamma subunit, activation of NF kappa B, and enhanced release of TNF-alpha and IL-1ra (8). Macrophage Dectin-2 alpha is upregulated in vivo by inflammatory stimuli and UV-B irradiation (6). It mediates the breaking of UV-induced tolerance by interacting with CD4⁺CD25⁺ regulatory T cells which then induce dendritic cells to release IL-4, IL-10, and TGF-beta (9). Within the ECD, mouse Dectin-2 alpha shares 71% aa sequence identity with human and bovine Dectin-2.

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
2. Kanazawa, N. et al. (2004) Immunobiology 209:179.
3. Fernandes, M.J. et al. (1999) Cancer Res. 59:2709.
4. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:11957.
5. Bonkobara, M. et al. (2005) Photochem. Photobiol. 81:944.
6. Taylor, P.R. et al. (2005) Eur. J. Immunol. 35:2163.
7. McGreal, E.P. et al. (2006) Glycobiology 16:422.
8. Sato, K. et al. (2006) J. Biol. Chem. 281:38854.
9. Aragane, Y. et al. (2003) J. Immunol. 171:3801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.