DEC2/SHARP1 Antibody (4H6)

Product Discontinued

DEC2/SHARP1 Antibody (4H6) Summary

• Immunogen: BHLHB3 (NP_110389, 203 a.a. ~ 282 a.a) partial recombinant protein with GST tag.CLERAGQKLEPLAYCVPVIQRTQPSAELAAENDTDTDSGYGGEAEARPDREKGKGAGASRVTIKQEPPGEDSPAPKRMKL
• Specificity: BHLHB3 (4H6)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BHLHE41
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DEC2/SHARP1 Antibody (4H6)

• basic helix-loop-helix domain containing, class B, 3
• basic helix-loop-helix family, member e41
• BHLHB3
• BHLHB3class E basic helix-loop-helix protein 41
• BHLHE41
• Class B basic helix-loop-helix protein 3
• DEC2
• DEC2Enhancer-of-split and hairy-related protein 1
• Differentially expressed in chondrocytes protein 2
• SHARP-1
• SHARP1hDEC2

Background

Human DEC1 is a 412 amino acid, basic helix-loop-helix (bHLH) containing protein that is involved in the control of proliferation and/or differentiation of several cell types including nerve cells, fibroblasts and chondrocytes. The bHLH region of DEC1 is structurally similar to the bHLH regions of the mammalian HES family, Drosophila hairy, and Enhancer of split m7. DEC1 is a novel direct target for cAMP in a wide range of cells, and is involved in the control of gene expression in cAMP-activated cells. DEC2, also known as SHARP1, is highly expressed in skeletal muscle and brain. The gene encoding human DEC2 maps to chromosome 12p11.23-p12.1. DEC1 and DEC2 play a role in regulating the mammalian molecular clock by suppressing the transcription of specific clock genes. Both DEC1 and DEC2 are detected in the suprachiasmimc nucleus in a circadian fashion. Brief light impulses induce the expression of DEC1 in a phase-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DEC2/SHARP1 Antibody (H00079365-M01)(2)

Publications using H00079365-M01

• Huang Y, Kesselman D, Kizub D et al. Phospho-deltaNp63alpha/microRNA feedback regulation in squamous carcinoma cells upon cisplatin exposure. Cell Cycle 2013-02-15 [PMID: 23343772] (WB, Human)
• Lecomte V, Meugnier E, Euthine V et al. A new role for sterol regulatory element binding protein 1 transcription factors in the regulation of muscle mass and muscle cell differentiation. Mol Cell Biol 30(5):1182-98. 2010-03-01 [PMID: 20028734]

Bioinformatics

• Gene Symbol: BHLHE41
• Entrez:
  • 79365(Human)
• OMIM:
  • 606200(Human)
• Uniprot:
  • NP_110389(Human)