DEC-205/CD205 Antibody (HD30) - BSA Free

Flow Cytometry: Renal Cell Carcinoma (gp200) Antibody (HD30) [NBP1-41003] - KM-H2 cells.

Flow Cytometry: Renal Cell Carcinoma (gp200) Antibody (HD30) [NBP1-41003] - KM-H2 cells stained with Mouse anti Human CD205

Product Discontinued

DEC-205/CD205 Antibody (HD30) - BSA Free Summary

• Immunogen: Purified extracellular domain of human CD205-IgG1 Fc fusion protein.
• Specificity: This antibody specifically recognises CD205, otherwise known as DEC205, a type I transmembrane glycoprotein and member of the macrophage mannose receptor family of C-type lectins, predominantly expressed by human dendritic cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LY75
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: DEC-205/CD205 Antibody (HD30) has been validated for IHC-Fr, but not for IHC-P.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for DEC-205/CD205 Antibody (HD30) - BSA Free

• CD205
• CLEC13B
• C-type lectin domain family 13 member B
• DEC205
• DEC-205
• GP200-MR6
• Ly75
• Ly-75
• Lymphocyte antigen 75

Background

gp200 is a surface membrane glycoprotein expressed on human embryonal carcinoma and is a malignant stem cell of testicular tumors. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Of other normal tissues examined, the gp200 is also localized along the luminal surfaces of breast lobules and ducts, the luminal surface of the epididymal tubular epithelium, within the cytoplasm of parathyroid parenchymal cells, and focally within the colloid of thyroid follicles. Thirty one other normal tissues do not express similar or cross reacting antigens. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LY75
• Entrez:
  • 4065(Human)
• Uniprot:
  • O60449()