DDX56 Antibody (6B9) - Azide and BSA Free

Western Blot: DDX56 Antibody (6B9) [H00054606-M03] - Analysis of DDX56 expression in Raw 264.7 (Cat # L024V1).

Western Blot: DDX56 Antibody (6B9) [H00054606-M03] - Analysis of DDX56 expression in HeLa (Cat # L013V1).

Western Blot: DDX56 Antibody (6B9) [H00054606-M03] - Analysis of DDX56 expression in PC-12 (Cat # L012V1).

Western Blot: DDX56 Antibody (6B9) [H00054606-M03] - Analysis of DDX56 expression in HepG2 (Cat # L019V1).

Western Blot: DDX56 Antibody (6B9) [H00054606-M03] - Analysis of DDX56 expression in NIH/3T3 (Cat # L018V1).

ELISA: DDX56 Antibody (6B9) [H00054606-M03] - Detection limit for recombinant GST tagged DDX56 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 6B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DDX56 Antibody (6B9) - Azide and BSA Free Summary

• Immunogen: DDX56 (NP_061955, 450 a.a. ~ 547 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. IKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPPALRGLVRPHKKRKKLSSSCRKAKRAKSQNPLRSFKHKGKKFRPTAKPS
• Specificity: DDX56 - DEAD (Asp-Glu-Ala-Asp) box polypeptide 56 (6B9)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDX56
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DDX56 Antibody (6B9) - Azide and BSA Free

• ATP-dependent 61 kDa nucleolar RNA helicase
• DDX26
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 56
• DEAD box protein 21
• DEAD box protein 56
• DEAD-box RNA helicase
• EC 3.6.1
• EC 3.6.4.13,61-kd nucleolar helicase
• NOH61DDX21
• probable ATP-dependent RNA helicase DDX56
• putative nucleolar RNA helicase

Background

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX56 Antibody (H00054606-M03)(1)

Publication using H00054606-M03

• Moore HM, Bai B, Boisvert FM et al. Quantitative Proteomics and Dynamic Imaging of the Nucleolus Reveal Distinct Responses to UV and Ionizing Radiation. Mol Cell Proteomics. 2011-07-21 [PMID: 21778410]

Bioinformatics

• Gene Symbol: DDX56
• Entrez:
  • 54606(Human)
• Uniprot:
  • NP_061955(Human)