DDX3 Antibody (11F11D10) - Azide and BSA Free

Western Blot: DDX3 Antibody (11F11D10) - Azide and BSA Free [NBP2-80692] - Analysis of human DDX3 using A) a partial recombinant protein (amino acids 24-203) and B) 293 lysate probed with DDX3 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.

Flow Cytometry: DDX3 Antibody (11F11D10) - Azide and BSA Free [NBP2-80692] - Analysis of DDX3 in human MCF-7 and HeLa cells using DDX3 antibody at 1 ug/10^6 cells. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: 11F11D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

DDX3 Antibody (11F11D10) - Azide and BSA Free Summary

• Immunogen: A partial recombinant protein containing amino acids 24-203 of human DDX3 was used as immunogen for this antibody.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDX3X
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 ug/10^6 cells
  • Western Blot 2-5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for DDX3 Antibody (11F11D10) - Azide and BSA Free

• DBXCAP-Rf
• DDX14
• DDX3DEAD/H box-3
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• DEAD box protein 3, X-chromosomal
• DEAD box, X isoform
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3
• EC 3.6.1
• EC 3.6.4.13
• helicase like protein 2
• Helicase-like protein 2
• HLP2ATP-dependent RNA helicase DDX3X

Background

DDX3 is a conserved RNA helicase ubiquitously expressed in the cytoplasm. A 73kD protein and member of the DEAD-box family, DDX3 appears to have important regulatory activities in a variety of cell programs including growth and cell cycle control, apoptosis, and anti-viral signaling pathways. HIV and HCV co-opt the DDX3 molecule as an essential host factor for their replication. Additionally DDX3 appears to recognize and sense viral RNA, interacts with IPS-1 dependent signaling pathway molecules, and TRIF, an adaptor molecule in TLR3 and TLR4 signaling pathways, as well as ZBP1 which also is involved in upregulation of IFNb by HCMV. Loss of DDX3 may lead to enhanced proliferation and anti-apoptotic programs -pathways that promote a role for DDX3 in tumorigenesis. The availability of antibody specific for DDX3 provides opportunity to further detail its potential role in anti-viral immunity as well as serving as a novel target for intervention.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX3X