DDX19B Antibody - BSA Free

Western Blot: DDX19B Antibody [NBP2-16127] - Sample (30 ug of whole cell lysate) A: A549 B: Hep G2 10% SDS PAGE gel, diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: DDX19B Antibody [NBP2-16127] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

• Reactivity: Hu, Pm
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DDX19B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DDX19B Antibody - BSA Free (NBP2-16127) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human DDX19B. The exact sequence is proprietary.
• Localization: Cytoplasm; Nucleus; nuclear pore complex; Nucleus membrane
• Predicted Species: Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX19B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for DDX19B Antibody - BSA Free

• ATP-dependent RNA helicase DDX19
• DBP5ATP-dependent RNA helicase DDX19B
• DDX19DEAD-box protein 5
• DEAD (Asp-Glu-Ala-As) box polypeptide 19
• DEAD (Asp-Glu-Ala-As) box polypeptide 19B
• DEAD box protein 19B
• DEAD box RNA helicase DEAD5
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog)
• DEAD-box RNA helicase DEAD5
• EC 3.6.1
• EC 3.6.4.13
• RNAh
• yeast Dbp5 homolog

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DDX19B
• Uniprot:
  • Q9UMR2(Human)