DDT Antibody

Images

 
Western Blot: DDT Antibody [NBP1-33461] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with DDT antibody [N1C3] diluted at 1:500.
Immunohistochemistry-Paraffin: DDT Antibody [NBP1-33461] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.
Western Blot: DDT Antibody [NBP1-33461] - Sample (30 ug of whole cell lysate) A: Raji 15% SDS PAGE, antibody diluted at 1:500.
Western Blot: DDT Antibody [NBP1-33461] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with DDT antibody [N1C3] diluted at 1:500

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DDT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DDT. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Rhesus Macaque (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Pig (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for DDT Antibody

  • DDCT
  • D-Dopachrome Decarboxylase
  • D-dopachrome tautomeraseDDCT
  • DDT
  • DOPD
  • EC 4.1.1.84
  • MIF2
  • Phenylpyruvate Tautomerase II

Background

D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DDT
Entrez
Uniprot