DDR2 Antibody (290804) [Alexa Fluor® 647]

HEK293 human embryonic kidney cell line transfected with human DDR2 (filled histogram) or irrelevant transfectants (open histogram) were stained with Mouse Anti-Human DDR2 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB25381R). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 290804
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

DDR2 Antibody (290804) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DDR2
  Gln24-Arg399
  Accession # Q16832
• Specificity: Detects human DDR2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DDR2 Antibody (290804) [Alexa Fluor® 647]

• CD167 antigen-like family member B
• CD167b antigen
• DDR2
• Discoidin domain receptor 2
• discoidin domain receptor family, member 2
• discoidin domain receptor tyrosine kinase 2
• discoidin domain-containing receptor 2
• EC 2.7.10
• EC 2.7.10.1
• hydroxyaryl-protein kinase
• migration-inducing gene 16 protein
• neurotrophic tyrosine kinase receptor related 3
• Neurotrophic tyrosine kinase, receptor-related 3
• NTRKR3cell migration-inducing protein 20
• Receptor protein-tyrosine kinase TKT
• TKT
• TKTMIG20a
• Trk3
• TYRO10
• Tyro-10
• Tyrosine-protein kinase TYRO10
• tyrosylprotein kinase

Background

DDR2, also known as TYR010 and TKT, is a widely expressed 130 kDa type I transmembrane glycoprotein belonging to the discoidin-like domain containing subfamily of receptor tyrosine kinases (1). Mature human DDR2 consists of a 378 amino acid (aa) extracellular domain (ECD) that includes the discoidin-like domain, a 22 aa transmembrane segment, and a 434 aa cytoplasmic domain that includes the kinase domain (2). Within the ECD, human DDR2 shares 53% aa sequence identity with DDR1 and 97% aa sequence identity with mouse DDR2. The discoidin-like domain mediates DDR2 interactions with collagens I, III, and X (3‑5). Collagens II and V are less efficacious ligands (3). DDR2 selectively recognizes the triple helical structure of collagen compared to monomeric or denatured collagen (3, 5, 6). Within collagen II, the D2 period is required for DDR2 binding, and the D1 period is additionally required to trigger DDR2 autophosphorylation (6). The ECD of DDR2 exists as a non-covalent dimer in solution, and dimerization of the receptor greatly enhances collagen binding (4, 7). DDR2 interaction with collagen I inhibits collagen fibrillogenesis and alters collagen fiber morphology (7). Ligand binding induces DDR2 autophosphorylation in the cytoplasmic domain (3, 5, 8), which promotes associations with Shc and Src (9). In addition to the above mechanism, DDR2 exhibits a distinct interaction with collagen X. A region other than the discoidin-like domain of DDR2 recognizes the non-helical NC1 domain of collagen X, and this interaction does not lead to receptor autophosphorylation (5). Activation of DDR2 by collagen induces upregulation of MMP-1, -2, and -13 as well as DDR2 itself (3, 8, 10). DDR2 is implicated in collagenous matrix destruction and cell invasiveness (8, 10). DDR2 is also upregulated in several pathological conditions, including hepatic fibrosis following injury, rheumatoid and osteoarthritis, and smooth muscle cell hyperplasia (8, 10‑12).

1. Vogel, W.F. et al. (2006) Cell. Signal. 18:1108.
2. Karn, T. et al. (1993) Oncogene 8:3433.
3. Vogel, W. et al. (1997) Mol. Cell 1:13.
4. Leitinger, B. (2003) J. Biol. Chem. 278:16761.
5. Leitinger, B. and A.P.L Kwan (2006) Matrix Biol. 25:355.
6. Leitinger, B. et al. (2004) J. Mol. Biol. 344:993.
7. Mihai, C. et al. (2006) J. Mol. Biol. 361:864.
8. Olaso, E. et al. (2001) J. Clin. Invest. 108:1369.
9. Ikeda, K. et al. (2002) J. Biol. Chem. 277:19206.
10. Xu, L. et al. (2005) J. Biol. Chem. 280:548.
11. Wang, J. et al. (2002) J. Autoimmun. 19:161.
12. Ferri, N. et al. (2004) Am. J. Pathol. 164:1575.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDR2 Antibody (FAB25381R)(1)

Publication using FAB25381R

• L Man, N Lustgarten, E Kallinos, Z Redhead-La, S Liu, B Schattman, D Redmond, K Hancock, N Zaninovic, G Schattman, Z Rosenwaks, D James Comparison of Human Antral Follicles of Xenograft versus Ovarian Origin Reveals Disparate Molecular Signatures Cell Rep, 2020-08-11;32(6):108027. 2020-08-11 [PMID: 32783948] (Flow Cytometry, ICC, IHC, Human)

Bioinformatics

• Gene Symbol: DDR2
• Uniprot:
  • Q16832()