DDR2 Antibody (290804) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 290804
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

DDR2 Antibody (290804) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DDR2
  Gln24-Arg399
  Accession # Q16832
• Specificity: Detects human DDR2 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDR2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Flow Cytometry
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for DDR2 Antibody (290804) [CoraFluor™ 1]

• CD167 antigen-like family member B
• CD167b antigen
• DDR2
• Discoidin domain receptor 2
• discoidin domain receptor family, member 2
• discoidin domain receptor tyrosine kinase 2
• discoidin domain-containing receptor 2
• EC 2.7.10
• EC 2.7.10.1
• hydroxyaryl-protein kinase
• migration-inducing gene 16 protein
• neurotrophic tyrosine kinase receptor related 3
• Neurotrophic tyrosine kinase, receptor-related 3
• NTRKR3cell migration-inducing protein 20
• Receptor protein-tyrosine kinase TKT
• TKT
• TKTMIG20a
• Trk3
• TYRO10
• Tyro-10
• Tyrosine-protein kinase TYRO10
• tyrosylprotein kinase

Background

DDR2 (discoidin domain receptor family, member 2) is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/ threonine (STK) kinase catalytic domains. Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDR2