DDR1 Knockout HeLa Cell Lysate

Product Discontinued

DDR1 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,47 bp insertion in exon and Insertion of the selection cassette in exon
• Gene: DDR1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for DDR1 Knockout HeLa Cell Lysate

• CAK
• CD167 antigen-like family member A
• CD167a antigen
• CD167a
• DDR
• DDR1
• discoidin domain receptor family, member 1
• discoidin domain receptor tyrosine kinase 1
• Discoidin receptor tyrosine kinase
• EC 2.7.10
• EC 2.7.10.1
• EDDR1
• ENTRK4
• Epithelial discoidin domain receptor 1
• HGK2
• MCK10
• MCK-10
• NTRK4
• Protein-tyrosine kinase 3A
• PTK3A protein tyrosine kinase 3A
• PTK3A
• receptor, type 4
• RTK6
• TRK E
• TrkE

Background

Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

FAQs for DDR1 Lysate (NBP2-66327). (Showing 1 - 1 of 1 FAQ).

1. I was wondering if you offer, or are planning on offering, any antibodies targeted to specific phosphorylation sites on the receptor tyrosine kinase DDR1.
   • At this time we do not offer and DDR1 antibodies specific for certain phosphorylation states.

Bioinformatics

• Gene Symbol: DDR1