DDR1 Antibody (2G4E12) - BSA Free

Western Blot: DDR1 Antibody (2G4E12) [NBP1-28861] - analysis using anti-DDR1 monoclonal antibody against truncated MBP-DDR1 recombinant protein(1).

Immunohistochemistry-Paraffin: DDR1 Antibody (2G4E12) [NBP1-28861] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Immunohistochemistry-Paraffin: DDR1 Antibody (2G4E12) [NBP1-28861] - Staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

Product Discontinued

DDR1 Antibody (2G4E12) - BSA Free Summary

• Immunogen: Purified recombinant fragment of DDR1 (aa602-681) expressed in E. Coli.
• Specificity: Specific for DDR1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDR1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:500-1:2000

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for DDR1 Antibody (2G4E12) - BSA Free

• CAK
• CD167 antigen-like family member A
• CD167a antigen
• CD167a
• DDR
• DDR1
• discoidin domain receptor family, member 1
• discoidin domain receptor tyrosine kinase 1
• Discoidin receptor tyrosine kinase
• EC 2.7.10
• EC 2.7.10.1
• EDDR1
• ENTRK4
• Epithelial discoidin domain receptor 1
• HGK2
• MCK10
• MCK-10
• NTRK4
• Protein-tyrosine kinase 3A
• PTK3A protein tyrosine kinase 3A
• PTK3A
• receptor, type 4
• RTK6
• TRK E
• TrkE

Background

discoidin domain receptor tyrosine kinase 1. Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDR1 Antibody (NBP1-28861)(1)

Publication using NBP1-28861

• Filliol A, Saito Y, Nair A et al. Opposing roles of hepatic stellate cell subpopulations in hepatocarcinogenesis Nature 2022-10-01 [PMID: 36198802] (IF/IHC, Human)
  
  Details:
  Dilution used in IHC 1:500

FAQs for DDR1 Antibody (NBP1-28861). (Showing 1 - 3 of 3 FAQ).

1. I was wondering if you offer, or are planning on offering, any antibodies targeted to specific phosphorylation sites on the receptor tyrosine kinase DDR1.
   • At this time we do not offer and DDR1 antibodies specific for certain phosphorylation states.
2. Please inform us the positive control of this item NBP1-28861.
   • Positive Controls: Normal - brain, lung, testes or kidney. Cancerous – colon, testes, bladder 
3. Can you please confirm how many slides I can test for IHC-P?
   • Tests: Its dilution for IHC-P is 1:200, so it will be good for about 125-150 slides if you use 150ul of diluted antibody/slide.

Bioinformatics

• Gene Symbol: DDR1