DDIT4 Antibody

Western Blot: DDIT4 Antibody [NBP2-80416] - Analysis of DDIT4 on Hela (1) and K562 (2) cell lysates using anti-DDIT4 antibody at 1/200 dilution.

Immunocytochemistry/Immunofluorescence: DDIT4 Antibody [NBP2-80416] - Staining DDIT4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP2-80416] - Analysis in human tonsil tissue using DDIT4 antibody. Counter stained with hematoxylin.

Flow Cytometry: DDIT4 Antibody [NBP2-80416] - Analysis of A549 cells with DDIT4 antibody at 1/100 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).

Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP2-80416] - Analysis in human colon cancer tissue using DDIT4 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP2-80416] - Analysis in human breast tissue using DDIT4 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP2-80416] - Analysis in mouse colon tissue using DDIT4 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: DDIT4 Antibody [NBP2-80416] - Analysis in rat epididymis tissue using DDIT4 antibody. Counter stained with hematoxylin.

Product Discontinued

DDIT4 Antibody Summary

• Immunogen: Synthetic peptide within C-terminal human DDIT4. (SwissProt: Q9NX09 Human;SwissProt: Q9D3F7 Mouse;SwissProt: Q8VHZ9 Rat)
• Localization: Mitochondrion. Cytosol.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDIT4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1X TBS(pH7.4), 0.5%BSA, 50%Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for DDIT4 Antibody

• Dig2
• DNA-damage-inducible transcript 4
• FLJ20500
• HIF-1 responsive RTP801 (RTP801)
• Protein regulated in development and DNA damage response 1
• REDD-1DNA damage-inducible transcript 4 protein
• REDD1HIF-1 responsive protein RTP801
• RTP801RP11-442H21.1

Background

REDD1 (regulated in development and DNA damage response 1) is a direct transcriptional target of HIF. It plays a role in the TSC/mTOR pathway as a regulator upstream of the TSC1/2 complex. In response to both hypoxia and energy stress, REDD1 inhibits mTOR activity in a TSC-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDIT4