DCUN1D1 Antibody - Azide and BSA Free

Western Blot: DCUN1D1 Antibody [NBP2-96995] - Anti-DCUN1D1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa

Immunoprecipitation: DCUN1D1 Antibody [NBP2-96995] - DCUN1D1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-DCUN1D1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DCUN1D1 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 26 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DCUN1D1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human DCUN1D1 (Accession#: NP_065691.2; Met1-Val259)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DCUN1D1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for DCUN1D1 Antibody - Azide and BSA Free

• DCN1
• DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae)
• DCN1-like protein 1
• DCNL1
• DCUN1D1
• DCUN1L1
• DCUN1L1DCUN1 domain-containing protein 1
• Defective in cullin neddylation protein 1-like protein 1
• RP42 homolog
• RP42
• RP42Squamous cell carcinoma-related oncogene
• SCCRO
• SCCROSCRO
• Tes3

Background

DCUN1D1 may contribute to neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes. Neddylation of cullins play an essential role in the regulation of SCF-typecomplexes activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCUN1D1