Dcp1a Antibody

Western Blot: Dcp1a Antibody [NB100-2830] - Staining of lysate of cell line A431 (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Dcp1a Antibody Summary

• Description: Novus Biologicals Goat Dcp1a Antibody (NB100-2830) is a polyclonal antibody validated for use in WB and ELISA. Anti-Dcp1a Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QVLTKNKDNHN corresponding to C-Terminus according to NP_060873.3.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DCP1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:6000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 75 kDa band observed in human lysates of A431 and HeLa (calculated MW of 63.3 kDa band according to NP_060873.3).
• Control:

  Dcp1a Overexpression Lysate
  A-431 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Dcp1a Antibody

• DCP1 decapping enzyme homolog A (S. cerevisiae)
• decapping enzyme hDcp1a
• EC 3.-
• FLJ21691
• mRNA-decapping enzyme 1A
• Nbla00360
• putative protein product of Nbla00360
• Smad4-interacting transcriptional co-activator
• SMAD4IP1
• SMIFHSA275986
• Transcription factor SMIF

Background

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Dcp1a Antibody (NB100-2830)(1)

Publication using NB100-2830

• Cougot N, Babajko S, Seraphin B. Cytoplasmic foci are sites of mRNA decay in human cells. J Cell Biol 2004-04-01 [PMID: 15067023]

Bioinformatics

• Gene Symbol: DCP1A
• Entrez:
  • 55802(Human)
  • 75901(Mouse)
• Uniprot:
  • NP_060873.3(Human)