DC-SIGNR/CD299/CLEC4M Antibody (120604) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: Flow
• Clone: 120604
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

DC-SIGNR/CD299/CLEC4M Antibody (120604) [Alexa Fluor® 594] Summary

• Additional Information: Clone 120604 was used by HLDA to establish CD designation
• Immunogen: NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC‑SIGNR/CD299
  Accession # Q9H2X3
• Specificity: Detects human DC‑SIGNR/CD299.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC4M
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DC-SIGNR/CD299/CLEC4M Antibody (120604) [Alexa Fluor® 594]

• CD209L
• CD209L1
• CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2
• CD299 antigenMGC129964
• CD299
• CLEC4M
• C-type lectin domain family 4, member M
• DC-SIGN2
• DC-SIGN2CD209 antigen-like protein 1
• DCSIGNR
• DC-SIGNR
• DC-SIGNRDC-SIGN-related protein
• DCSIGNRMGC47866
• Dendritic cell-specific ICAM-3-grabbing non-integrin 2
• HP10347
• liver/lymph node-specific ICAM-3 grabbing non-integrin
• Liver/lymph node-specific ICAM-3-grabbing non-integrin
• LSIGN
• L-SIGN
• LSIGNC-type lectin domain family 4 member M
• mannose binding C-type lectin DC-SIGNR

Background

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN or CD299) and DC-SIGN related protein (DC-SIGNR, DC-SIGN2, L-SIGN or CD209L) are type II membrane proteins that are mannose-specific calcium-dependent (C-type) lectins. The two proteins share 77% amino acid identity. DC-SIGN mediates interactions between dendritic cells (DCs) and T cells. Both DC-SIGN and DC-SIGNR have been shown to bind HIV, hepatitis C glycoproteins, Ebola virus glycoproteins and the cellular adhesion protein ICAM-3 (1‑4). DC-SIGN and DC-SIGNR appear to selectively recognize and bind viral proteins containing a large portion of high-mannose oligosaccharides (5). Though DC-SIGN and DC-SIGNR are found on the same chromosome, they are not expressed in the same tissue. DC-SIGN is expressed solely on Dendritic cells while DC-SIGNR is found on endothelial cells in the liver and lymph node sinuses and in a significant portion of capillary endothelial cells in term placenta (1, 4).

1. Pohlmann, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670.
2. Pohlmann, S. et al. (2003) J. Virol. 77:4070.
3. Simmons, L.G. et al. (2003) J. Virol. 77:1337.
4. Bahirova, A.A. et al. (2001) J. Exp. Med. 193:671.
5. Feinberg, H. et al. (2001) Science 294:2163.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for DC-SIGNR/CD299/CLEC4M Antibody (FAB162T) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/13/2018

Summary

• Application: Flow Cytometry
• Sample Tested: DC-SIGNR transfected HEK293T
• Species: Human
• Lot: 1460464

Comments

• Comments: HEK293T cells were transfected with pcDNA3.DC-SIGNR and were analyzed by flow cytometry 48hrs post-transfections. 1/500 dilution, 2E5 cells.

Bioinformatics

• Gene Symbol: CLEC4M
• Uniprot:
  • Q9H2X3()