DBF4 Antibody (24D8) - BSA Free

Western Blot: DBF4 Antibody (24D8) [NBP3-26632] - Positive Western Blot detected in: PC3 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, U937 whole cell lysate. All lanes: DBF4 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 77, 27 kDa Observed band size: 72-95 kDa

Immunocytochemistry/Immunofluorescence: DBF4 Antibody (24D8) [NBP3-26632] - Staining of A549 cell with DBF4 Antibody (24D8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 551-conjugated Goat Anti-Rabbit IgG (H+L).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 24D8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DBF4 Antibody (24D8) - BSA Free Summary

• Description: Novus Biologicals Rabbit DBF4 Antibody (24D8) - BSA Free (NBP3-26632) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human DBF4 [UniProt Q9UBU7]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DBF4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DBF4 Antibody (24D8) - BSA Free

• ASKchif
• DBF4 homolog (S. cerevisiae)
• DBF4ACHIF
• DBF4-type zinc finger-containing protein 1
• ZDBF1DBF-type containing 1

Background

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DBF4