DBF4 Antibody (0S8I5)

Western Blot: DBF4 Antibody (0S8I5) [NBP3-15903] - Western blot analysis of extracts of various cell lines, using DBF4 Rabbit mAb (NBP3-15903) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

• Reactivity: Hu
• Applications: WB
• Clone: 0S8I5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

DBF4 Antibody (0S8I5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DBF4 (Q9UBU7). MNSGAMRIHSKGHFQGGIQVKNEKNRPSLKSLKTDNRPEKSKCKPLWGKVFYLDLPSVTISEKLQKDIKDLGGRVEEFLSKDISYLISNKKEAKFAQTLG
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DBF4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DBF4 Antibody (0S8I5)

• ASKchif
• DBF4 homolog (S. cerevisiae)
• DBF4ACHIF
• DBF4-type zinc finger-containing protein 1
• ZDBF1DBF-type containing 1

Background

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DBF4