DBC1/p30 Antibody (PCRP-KIAA1967-1D10) - Azide and BSA Free

Analysis of Protein Array containing more than 19,000 full-length human proteins using DBC1/p30 antibody (PCRP-KIAA1967-1D10) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human colon tumor stained with DBC1/p30 antibody (PCRP-KIAA1967-1D10) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Flow Cytometric Analysis of PFA-fixed HeLa cells. DBC1/p30 antibody (PCRP-KIAA1967-1D10) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: PCRP-KIAA1967-1D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

DBC1/p30 Antibody (PCRP-KIAA1967-1D10) - Azide and BSA Free Summary

• Description: Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human DBC1/p30 protein
• Localization: Nucleus.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCAR2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2ug/million cells
  • Immunohistochemistry-Paraffin 1-2ug/ml
• Application Notes: Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for DBC1/p30 Antibody (PCRP-KIAA1967-1D10) - Azide and BSA Free

• DBC.1
• DBC1deleted in breast cancer 1
• DBC-1p30 DBC protein
• Deleted in breast cancer gene 1 protein
• KIAA1967
• NET35
• p30 DBC
• p30DBC

Background

DBC1 inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediatedapoptosis. Inhibits SUV39H1 methyltransferase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CCAR2