DAZ3 Antibody

Western Blot: DAZ3 Antibody [NBP2-16103] - Sample (30 ug of whole cell lysate). A: Hela. 10% SDS PAGE. Diluted at 1:500

Immunocytochemistry/Immunofluorescence: DAZ3 Antibody [NBP2-16103] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DAZ3 antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: DAZ3 Antibody [NBP2-16103] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Product Discontinued

DAZ3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human DAZ3. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DAZ3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for DAZ3 Antibody

• deleted in azoospermia 3
• deleted in azoospermia protein 3
• MGC126441
• pDP1679

Background

This gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains one copy of the 10.8 kb repeat. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: DAZ3
• Uniprot:
  • Q9NR90(Human)