![Western Blot: DARC Antibody (2C3) [NBP2-75197] - Western Blot using anti-DARC antibody 2C3 (Ab00893) Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (NBP2-75197) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. NBP2-75197 successfully detected DARC in human liver lysate.](http://images.novusbio.com/fullsize2/DARC-Antibody-(2C3)-Western-Blot-NBP2-75197-img0001.jpg "Western Blot: DARC Antibody (2C3) [NBP2-75197] - Western Blot using anti-DARC antibody 2C3 (Ab00893) Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (NBP2-75197) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. NBP2-75197 successfully detected DARC in human liver lysate.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, B/N, ICC/IF |
| Clone | 2C3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | CHO cells transfected with cDNA encoding the Duffy glycoprotein. |
| Specificity | The antibody binds specifcally to the pentapeptide FEDVW of the Duffy receptor. |
| Isotype | IgG Kappa |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | ACKR1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Diseases for DARC Antibody (NBP2-75197)Discover more about diseases related to DARC Antibody (NBP2-75197).
| Pathways for DARC Antibody (NBP2-75197)View related products by pathway.
|
PTMs for DARC Antibody (NBP2-75197)Learn more about PTMs related to DARC Antibody (NBP2-75197).
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ACKR1 |
![Immunofluorescence: DARC Antibody (2C3) [NBP2-75197] - Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 (NBP2-75197) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75197, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize2/DARC-Antibody-(2C3)-Immunofluorescence-NBP2-75197-img0002.jpg "Immunofluorescence: DARC Antibody (2C3) [NBP2-75197] - Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 (NBP2-75197) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75197, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: RHD Antibody [NBP2-84247] - Host: Rabbit. Target Name: RHD. Sample Type: Human Fetal Liver. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize2/RHD-Antibody-Western-Blot-NBP2-84247-img0004.jpg)
![Western Blot: RHD Antibody [NBP2-84247] - WB Suggested Anti-RHD Antibody. Titration: 1.0 ug/ml. Positive Control: RPMI-8226 Whole Cell.RHD is strongly supported by BioGPS gene expression data to be expressed in RPMI 8226](http://images.novusbio.com/fullsize2/RHD-Antibody-Western-Blot-NBP2-84247-img0001.jpg)
![Western Blot: RHD Antibody [NBP2-84247] - Host: Rabbit. Target Name: RHD. Sample Type: 293T. Antibody Dilution: 1.0ug/mlRHD is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells](http://images.novusbio.com/fullsize2/RHD-Antibody-Western-Blot-NBP2-84247-img0002.jpg)
![Western Blot: RHCE Antibody [NBP1-69668] - This Anti-RHCE antibody was used in Western Blot of MCF7 tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/fullsize/RHCE Antibody-Western Blot-NBP1-69668-img0001.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Western blot image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/GAPDH-Antibody-(1D4)-Western-Blot-NB300-221-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - Analysis of of HeLa cells stained with GAPDH antibody, dilution 1:100 (Green). DAPI staining of nuclear DNA (Blue). The GAPDHantibody produces strong cytoplasmic staining of healthy cells.](http://images.novusbio.com/fullsize2/GAPDH-Antibody-(1D4)-Immunocytochemistry-Immunofluorescence-NB300-221-img0009.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.](http://images.novusbio.com/fullsize2/GAPDH-Antibody-(1D4)-Western-Blot-NB300-221-img0008.jpg)
![Western Blot: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Analysis of Haptoglobin on different cells lysates using anti-Haptoglobin antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: HepG2](http://images.novusbio.com/fullsize2/Haptoglobin Antibody (JM10-79)-Western Blot-NBP2-66881-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Staining Haptoglobin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Haptoglobin-Antibody-(JM10-79)-Immunocytochemistry-Immunofluorescence-NBP2-66881-img0003.jpg)
![Immunohistochemistry-Paraffin: Haptoglobin Antibody (JM10-79) [NBP2-66881] - Analysis of paraffin-embedded mouse lung tissue using anti-Haptoglobin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Haptoglobin-Antibody-(JM10-79)-Immunohistochemistry-Paraffin-NBP2-66881-img0007.jpg)
![Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - PGM1 monoclonal antibody (M01), clone 3B8-H4 Analysis of PGM1 expression in Jurkat.](http://images.novusbio.com/fullsize2/PGM1-Antibody-(3B8-H4)-Western-Blot-H00005236-M01-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of monoclonal antibody to PGM1 on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize2/PGM1-Antibody-(3B8-H4)-Immunocytochemistry-Immunofluorescence-H00005236-M01-img0008.jpg)
![Western Blot: PGM1 Antibody (3B8-H4) [H00005236-M01] - Analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi ( Cat # H00005236-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody (M01), clone 3B8-H4 (Cat # H00005236-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize2/PGM1-Antibody-(3B8-H4)-Western-Blot-H00005236-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Negative Control showing staining of paraffin embedded Mouse Adrenal Gland, with no primary antibody.](http://images.novusbio.com/fullsize2/DLC1-Antibody---Isoform-1-and-3-Immunohistochemistry-Paraffin-NB300-917-img0008.jpg)
![Immunohistochemistry-Paraffin: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Staining of paraffin embedded Mouse Adrenal Gland. Antibody at 4 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize2/DLC1-Antibody---Isoform-1-and-3-Immunohistochemistry-Paraffin-NB300-917-img0007.jpg)
![Immunofluorescence: DLC1 Antibody - Isoform 1 and 3 [NB300-917] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize2/DLC1-Antibody---Isoform-1-and-3-NB300-917-img0003.jpg)
![Western Blot: Glycophorin C Antibody [NBP1-84597] - Analysis in control (vector only transfected HEK293T lysate) and GYPC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/Glycophorin-C-Antibody-Western-Blot-NBP1-84597-img0007.jpg)
![Immunohistochemistry-Paraffin: Glycophorin C Antibody [NBP1-84597] - Staining in human bone marrow and pancreas tissues using anti-GYPC antibody. Corresponding GYPC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Glycophorin-C-Antibody-Immunohistochemistry-Paraffin-NBP1-84597-img0006.jpg)
![Immunohistochemistry-Paraffin: Glycophorin C Antibody [NBP1-84597] - Staining of human colon shows strong immunoreactivity in erythrocytes present in blood vessel and moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/Glycophorin-C-Antibody-Immunohistochemistry-Paraffin-NBP1-84597-img0002.jpg)
