DARC Antibody (2C3) - Chimeric - Azide and BSA Free

Western Blot: DARC Antibody (2C3) - Chimeric [NBP2-75197] - Western Blot using anti-DARC antibody 2C3 (Ab00893) Human liver lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 2C3 (NBP2-75197) at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. NBP2-75197 successfully detected DARC in human liver lysate.

Immunocytochemistry /Immunofluorescence: DARC Antibody (2C3) - Chimeric - Azide and BSA Free [NBP2-75197] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on coverslips, permeabilized with 0.15% Triton and stained with NBP2-75197 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-75197, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, B/N
• Clone: 2C3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

DARC Antibody (2C3) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: CHO cells transfected with cDNA encoding the Duffy glycoprotein.
• Specificity: The antibody binds specifcally to the pentapeptide FEDVW of the Duffy receptor.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ACKR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for DARC Antibody (2C3) - Chimeric - Azide and BSA Free

• ACKR1
• CCBP1
• CD234 antigen
• CD234
• DARC
• Dfy
• Duffy Antigen
• Duffy antigen/chemokine receptor
• Duffy blood group antigen
• Duffy blood group, chemokine receptor
• Fy Antigen
• Fy glycoprotein
• FYDuffy blood group
• Glycoprotein D
• GPD
• GPDWBCQ1
• GpFy
• Plasmodium vivax receptor

Background

DARC is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DARC Antibody (NBP2-75197)(1)

Publication using NBP2-75197

• Guo X, Khosraviani N, Raju S et Al. Endothelial ACKR1 is induced by neutrophil contact and down-regulated by secretion in extracellular vesicles Front Immunol 2023-04-21 [PMID: 37153544]

Bioinformatics

• Gene Symbol: ACKR1