DAPK3/ZIPK Antibody

Images

 
Western Blot: DAPK3/ZIPK Antibody [NBP1-76962] - Analysis of ZIP kinase in (A) HeLa and (B) Jurkat lysates with ZIP kinase antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: DAPK3/ZIPK Antibody [NBP1-76962] - Staining of ZIPK in Jurkat cells with ZIPK antibody at 5 ug/ml.
Western Blot: DAPK3/ZIPK Antibody [NBP1-76962] - Analysis of 50 ug of whole cell lysate from HeLa cells with anti-ZIP kinase (IN) at 1:1000 dilution.
Immunocytochemistry/ Immunofluorescence: DAPK3/ZIPK Antibody [NBP1-76962] - Jurkat cells with ZIP kinase antibody at 10 ug/ml.
Immunocytochemistry/ Immunofluorescence: DAPK3/ZIPK Antibody [NBP1-76962] - Immunofluorescence of ZIPK in Jurkat cells with ZIPK antibody at 10 ug/mL.
Immunocytochemistry: DAPK3/ZIPK Antibody [NBP1-76962] - Staining of ZIPK in Jurkat cells with ZIPK antibody at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, ICC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DAPK3/ZIPK Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase. The immunogen is located within amino acids 270 - 320 of ZIPK.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DAPK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunocytochemistry
Application Notes
ZIPK antibody can be used for detection of DNase II expression by Western blot at 1:500 to 1:1000 dilution. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
DAPK3/ZIPK Peptide (NBP1-76962PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DAPK3/ZIPK Antibody

  • DAP kinase 3
  • DAPK3
  • DAP-like kinase
  • death-associated protein kinase 3
  • Dlk
  • EC 2.7.11
  • EC 2.7.11.1
  • ZIP kinase isoform
  • ZIP
  • ZIPK
  • ZIPKFLJ36473
  • ZIP-kinase

Background

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase. ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol DAPK3
Entrez
Uniprot