DAPK3/ZIPK Antibody (729830)

Western blot shows lysates of A431 human epithelial carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human DAPK3/ZIPK Monoclonal Antibody (Catalog # MAB5290) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAPK3/ZIPK at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 729830
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DAPK3/ZIPK Antibody (729830) Summary

• Immunogen: E. coli-derived recombinant human DAPK3/ZIPK
  Arg347-Arg454
  Accession # O43293
• Specificity: Detects human DAPK3/ZIPK in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DAPK1, rhDAPK2, rhDRAK1, or rhDRAK2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DAPK3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DAPK3/ZIPK Antibody (729830)

• DAP kinase 3
• DAPK3
• DAP-like kinase
• death-associated protein kinase 3
• Dlk
• EC 2.7.11
• EC 2.7.11.1
• ZIP kinase isoform
• ZIP
• ZIPK
• ZIPKFLJ36473
• ZIP-kinase

Background

DAPK3 (Death-associated protein kinase 3; also ZIP kinase and DLK) is a 52 kDa member of the CAMK Ser/Thr kinase family, protein kinase superfamily of enzymes.  In general, mouse DAPK3 is nuclear while human DAPK3 is cytosolic.  DAPK3 is found in smooth muscle where it phosphorylates the regulatory light chain of myosin and stabilizes thick filament formation.  Human DAPK3 is 454 amino acids (aa) in length.  It contains one protein kinase domain (aa 134-275) and a C‑terminal Leu-zipper motif (aa 427-441) that mediates homo- and hetero-dimerization.  There is one 35 kDa alternate splice form that shows a 10 aa substitution for aa 313-454.  This form has both a nuclear and cytosolic localization. Over aa 347‑454, human DAPK3 is 65% aa identical to mouse DAPK3.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAPK3
• Uniprot:
  • O43293(Human)