DAPK3/ZIPK Antibody (729830) [Alexa Fluor® 594]

• Applications: WB
• Clone: 729830
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

DAPK3/ZIPK Antibody (729830) [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human DAPK3/ZIPK
  Arg347-Arg454
  Accession # O43293
• Specificity: Detects human DAPK3/ZIPK in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DAPK1, rhDAPK2, rhDRAK1, or rhDRAK2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

DAPK3 (Death-associated protein kinase 3; also ZIP kinase and DLK) is a 52 kDa member of the CAMK Ser/Thr kinase family, protein kinase superfamily of enzymes.  In general, mouse DAPK3 is nuclear while human DAPK3 is cytosolic.  DAPK3 is found in smooth muscle where it phosphorylates the regulatory light chain of myosin and stabilizes thick filament formation.  Human DAPK3 is 454 amino acids (aa) in length.  It contains one protein kinase domain (aa 134-275) and a C‑terminal Leu-zipper motif (aa 427-441) that mediates homo- and hetero-dimerization.  There is one 35 kDa alternate splice form that shows a 10 aa substitution for aa 313-454.  This form has both a nuclear and cytosolic localization. Over aa 347‑454, human DAPK3 is 65% aa identical to mouse DAPK3.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.