DAPK3/ZIPK Antibody (729830) [Alexa Fluor® 350]

• Reactivity: Hu
• Applications: WB
• Clone: 729830
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

DAPK3/ZIPK Antibody (729830) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human DAPK3/ZIPK
  Arg347-Arg454
  Accession # O43293
• Specificity: Detects human DAPK3/ZIPK in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) DAPK1, rhDAPK2, rhDRAK1, or rhDRAK2 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DAPK3/ZIPK Antibody (729830) [Alexa Fluor® 350]

• DAP kinase 3
• DAPK3
• DAP-like kinase
• death-associated protein kinase 3
• Dlk
• EC 2.7.11
• EC 2.7.11.1
• ZIP kinase isoform
• ZIP
• ZIPK
• ZIPKFLJ36473
• ZIP-kinase

Background

DAPK3 (Death-associated protein kinase 3; also ZIP kinase and DLK) is a 52 kDa member of the CAMK Ser/Thr kinase family, protein kinase superfamily of enzymes.  In general, mouse DAPK3 is nuclear while human DAPK3 is cytosolic.  DAPK3 is found in smooth muscle where it phosphorylates the regulatory light chain of myosin and stabilizes thick filament formation.  Human DAPK3 is 454 amino acids (aa) in length.  It contains one protein kinase domain (aa 134-275) and a C‑terminal Leu-zipper motif (aa 427-441) that mediates homo- and hetero-dimerization.  There is one 35 kDa alternate splice form that shows a 10 aa substitution for aa 313-454.  This form has both a nuclear and cytosolic localization. Over aa 347‑454, human DAPK3 is 65% aa identical to mouse DAPK3.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O43293(Human)