DAP3 Antibody

Images

 
Western Blot: DAP3 Antibody [NB100-1032] - Staining (0.3 ug/mL) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: DAP3 Antibody [NB100-1032] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Western Blot: DAP3 Antibody [NB100-1032] - Staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30 ug total protein per lane). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DAP3 Antibody Summary

Immunogen
Peptide with sequence NPSLLERHCAYL corresponding to C-Terminus according to NP_387506.1, NP_001186779.1, NP_001186780.1.
Localization
Mitochondrial
Specificity
This antibody is expected to recognize isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DAP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:164000
Control
HeLa Lysate (NB800-PC1)
DAP3 Lysate (NBL1-09715)
Publications
Read Publication using NB100-1032.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DAP3 Antibody

  • bMRP-10
  • DAP-3Death-associated protein 3
  • death associated protein 3
  • DKFZp686G12159,28S ribosomal protein S29, mitochondrial
  • FLJ12817
  • MGC126058
  • MGC126059
  • mitochondrial 28S ribosomal protein S29
  • MRP-S29Ionizing radiation resistance conferring protein
  • MRPS29S29mt

Background

Death Associated Protein 3 (DAP3) is a 46 kDa protein carrying a potential nucleotide binding motif (P-loop) at its N-terminal region. The protein is widely expressed, and the expression is upregulated during membrane-receptor mediated apoptosis. In interferon-gamma and Fas induced apoptosis, DAP3 acts as a positive mediator by functioning downstream of the receptor signaling complex and upstream of the effector caspases. However, the mechanism for its function is largely unknown. Interestingly, the N-terminal 230 amino acid fragment of DAP3 alone protects cells from Fas induced cell death. The same region of DAP3 also serves as a binding site for the glucocorticoid receptor and mediates receptor functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DAP3 Antibody (NB100-1032)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DAP3
Entrez
Uniprot