DAP3 Antibody

Western Blot: DAP3 Antibody [NB100-1032] - Staining (0.3 ug/mL) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: DAP3 Antibody [NB100-1032] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Western Blot: DAP3 Antibody [NB100-1032] - Staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30 ug total protein per lane). Antibody at 0.3 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: DAP3 Antibody [NB100-1032] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Immunohistochemistry-Paraffin: DAP3 Antibody [NB100-1032] - (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DAP3 Antibody Summary

• Description: Novus Biologicals Goat DAP3 Antibody (NB100-1032) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-DAP3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence NPSLLERHCAYL corresponding to C-Terminus according to NP_387506.1, NP_001186779.1, NP_001186780.1.
• Localization: Mitochondrial
• Specificity: This antibody is expected to recognize isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DAP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 1 ug/mL
• Application Notes: WB: Approx 40 kDa band observed in lysates of cell lines HeLa and HepG2 and in Human Kidney lysates (calculated MW of 41 kDa according to NP_001186779.1 and NP_001186780). Primary incubated 1 hour at room temperature.
• Control:

  HeLa Whole Cell Lysate
  DAP3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DAP3 Antibody

• bMRP-10
• DAP-3Death-associated protein 3
• death associated protein 3
• DKFZp686G12159,28S ribosomal protein S29, mitochondrial
• FLJ12817
• MGC126058
• MGC126059
• mitochondrial 28S ribosomal protein S29
• MRP-S29Ionizing radiation resistance conferring protein
• MRPS29S29mt

Background

Death Associated Protein 3 (DAP3) is a 46 kDa protein carrying a potential nucleotide binding motif (P-loop) at its N-terminal region. The protein is widely expressed, and the expression is upregulated during membrane-receptor mediated apoptosis. In interferon-gamma and Fas induced apoptosis, DAP3 acts as a positive mediator by functioning downstream of the receptor signaling complex and upstream of the effector caspases. However, the mechanism for its function is largely unknown. Interestingly, the N-terminal 230 amino acid fragment of DAP3 alone protects cells from Fas induced cell death. The same region of DAP3 also serves as a binding site for the glucocorticoid receptor and mediates receptor functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAP3 Antibody (NB100-1032)(1)

Publication using NB100-1032

• Kissil JL, Deiss LP, Bayewitch M et al. Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death. J Biol Chem 1995-11-17 [PMID: 7499268]

Bioinformatics

• Gene Symbol: DAP3
• Entrez:
  • 7818(Human)
• Uniprot:
  • NP_001186779.1(Human)
  • NP_001186780.1(Human)
  • NP_387506.1(Human)