DAP3 Antibody

Images

 
Western Blot: DAP3 Antibody [NB100-1032] - (1ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35ug protein in RIPA buffer). Level of knock-down ...read more
Immunocytochemistry/ Immunofluorescence: DAP3 Antibody [NB100-1032] - staining of MCF7 cells with 5ug/ml NB100-1032 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000.
Immunohistochemistry-Paraffin: DAP3 Antibody [NB100-1032] - Staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: DAP3 Antibody [NB100-1032] - (3ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot: DAP3 Antibody [NB100-1032] - HEK293 overexpressing DAP3 and probed with (mock transfection in first lane).
Immunocytochemistry/ Immunofluorescence: DAP3 Antibody [NB100-1032] - (0.5ug/ml) staining of guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson. Primary ...read more
Immunohistochemistry-Paraffin: DAP3 Antibody [NB100-1032] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

DAP3 Antibody Summary

Immunogen
Peptide with sequence NPSLLERHCAYL corresponding to C-Terminus according to NP_387506.1, NP_001186779.1, NP_001186780.1.
Localization
Mitochondrial
Specificity
This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1; NP_004623.1; NP_001186778.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DAP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunocytochemistry/Immunofluorescence 3 - 10 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
ICC: Mitochondrial staining of HeLa after in situ denaturation of the target protein using 3-4M guanidinium thiocyanate (patent pending). The subcellular localisation is in agreement with scientific literaturesscientific literature (PMID: 11162496, 20563667). The signal goes down upon knockdown of the DAP3 expression.
WB: Approx. 40 kDa band observed in HeLa lysates (calculated MW of 45.6 kDa band according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48 kDa is observed. This band is not observed in the non-transfected HEK293. The band fades in HeLa after si-RNA-mediated knock-down (including the fainter shoulder band at 37 kDa).
IHC: Paraffin embedded Human Tonsil and Brain (Cortex).
Immunofluorescence: Protein expression clearly seen within the nucleus.
Control
HeLa Lysate (NB800-PC1)
DAP3 Lysate (NBL1-09715)
Publications
Read Publication using NB100-1032.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DAP3 Antibody

  • bMRP-10
  • DAP-3Death-associated protein 3
  • death associated protein 3
  • DKFZp686G12159,28S ribosomal protein S29, mitochondrial
  • FLJ12817
  • MGC126058
  • MGC126059
  • mitochondrial 28S ribosomal protein S29
  • MRP-S29Ionizing radiation resistance conferring protein
  • MRPS29S29mt

Background

Death Associated Protein 3 (DAP3) is a 46 kDa protein carrying a potential nucleotide binding motif (P-loop) at its N-terminal region. The protein is widely expressed, and the expression is upregulated during membrane-receptor mediated apoptosis. In interferon-gamma and Fas induced apoptosis, DAP3 acts as a positive mediator by functioning downstream of the receptor signaling complex and upstream of the effector caspases. However, the mechanism for its function is largely unknown. Interestingly, the N-terminal 230 amino acid fragment of DAP3 alone protects cells from Fas induced cell death. The same region of DAP3 also serves as a binding site for the glucocorticoid receptor and mediates receptor functions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DAP3 Antibody (NB100-1032)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol DAP3
Entrez
Uniprot