DAP12 Antibody

Immunohistochemistry-Paraffin: DAP12 Antibody [NBP1-52376] - Analysis of anti-TYROBP / DAP12 antibody with human spleen at concentration 3 ug/ml.

Immunohistochemistry-Paraffin: DAP12 Antibody [NBP1-52376] - Analysis of anti-TYROBP / DAP12 antibody with human uterus at concentration 3 ug/ml.

• Reactivity: Hu, Bt, Ca, Eq, Pm, Pm, Pm, Rb, Sh
• Applications: ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DAP12 Antibody Summary

• Immunogen: Synthetic peptide C-KQRITETESPYQE from an internal region of human DAP12 (NP_003323.1; NP_937758.1).
• Epitope: C-KQRITETESPYQE from an internal region
• Specificity: Human DAP12. This antibody is expected to recognize both reported isoforms (NP_003323.1; NP_937758.1)
• Predicted Species: Rabbit (92%), Chimpanzee (100%), Sheep (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TYROBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunohistochemistry 3-6 ug/ml
  • Immunohistochemistry-Paraffin 3-6 ug/ml
  • Peptide ELISA
• Application Notes: IHC: NBP1-52376 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Use in FLOW reported in scientific literature (PMID: 31201275)..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Mouse (85%), Bovine (85%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DAP12 Antibody

• DAP12
• DAP12PLOSL
• DNAX-activation protein 12
• KARAP
• KARAPPLO-SL
• KAR-associated protein
• killer activating receptor associated protein
• Killer-activating receptor-associated protein
• TYRO protein tyrosine kinase binding protein
• TYRO protein tyrosine kinase-binding protein
• TYROBP

Background

The 32kDa dopamine and cyclic adenosine 3',5'-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAP12 Antibody (NBP1-52376)(1)

Publication using NBP1-52376

• Tecle E, Reynoso HS, Wang R, Gagneux P The female reproductive tract contains multiple innate sialic acid-binding immunoglobulin-like lectins (Siglecs) that facilitate sperm survival Electrophoresis 2019-06-14 [PMID: 31201275] (FLOW, Human)

Bioinformatics

• Gene Symbol: TYROBP
• Entrez:
  • 7305(Human)
• Uniprot:
  • O43914(Human)